| Field | Specification |
|---|---|
| Target | |
| Alternative names | DNA repair protein XRCC3; X-ray repair cross-complementing protein 3; XRCC3 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human XRCC3 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 25X84 is a rabbit monoclonal antibody that recognizes DNA repair protein XRCC3 in human, mouse and rat samples and is validated for Western blot and flow cytometry. The predicted molecular weight is 37.9 kDa, and the supplier reports an observed band at about 42 kDa.
XRCC3 is a RAD51 paralog that partners with RAD51C to form the CX3 complex, which acts in homologous recombination repair of DNA double-strand breaks. The complex has been linked to later steps of recombination, including processing of Holliday junctions, and XRCC3 is studied in DNA repair and responses to cross-linking agents.
| Target | DNA repair protein XRCC3 (gene XRCC3; UniProt O43542, human) |
|---|---|
| Clone | 25X84 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 37.9 kDa |
| Observed MW | 42 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human XRCC3.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places DNA repair protein XRCC3 in the nucleus, cytoplasm and mitochondrion. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
