| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human ANXA2 was used as the immunogen for the ANXA2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
ANXA2 Antibody / Annexin A2 is a anti-ANXA2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: ANXA2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS
Biological background
Structurally, Annexin A2 is a 339-amino-acid protein of approximately 39 kilodaltons containing four annexin repeats that mediate calcium-dependent phospholipid binding and a unique N-terminal domain that interacts with S100A10 (p11) to form a heterotetrameric complex. This complex localizes to the plasma membrane, cytoskeleton, and endosomal compartments, where it modulates vesicle fusion, cytoskeletal anchoring, and membrane curvature. Annexin A2 is expressed in endothelial, epithelial, and tumor cells, as well as macrophages and neurons.
The ANXA2 antibody is widely used in cell biology, vascular biology, and cancer research to study membrane organization, signal transduction, and fibrinolytic regulation. Western blot analysis detects a 39 kilodalton band corresponding to Annexin A2, while immunofluorescence shows cytoplasmic, membrane, and perinuclear localization. This antibody provides a powerful reagent for investigating calcium-dependent membrane remodeling and extracellular proteolytic activity.
Functionally, Annexin A2 regulates actin cytoskeleton rearrangement and vesicle trafficking, influencing endocytosis, exocytosis, and cell motility. At the cell surface, it promotes plasmin generation, contributing to fibrin degradation, angiogenesis, and tumor invasion. Dysregulation of ANXA2 expression is associated with cancer progression, thrombosis, and inflammatory disease. The ANXA2 antibody supports detailed studies of its role in cellular dynamics, extracellular matrix remodeling, and vascular homeostasis.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
