AP-1 Monoclonal Antibody

SKU:BHA12102021
Overview
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Anti-JUN antibody (Mouse, Monoclonal, IgG). Validated for Western Blot, Immunohistochemistry, Immunofluorescence. Reactive against Human, Mouse, Monkey targets.
Clonality Monoclonal
Host Species Mouse
Species Reactivity Human, Mouse, Monkey
Isotype IgG
Validated Applications Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
Purification Method Affinity Purification
Options selector
Catalog no. Size
BT-MCA0162-50UL 50 uL
BT-MCA0162-100UL 100 uL
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 50 uL, 100 uL
  • Lead time: In Stock at Manufacturer options listed in “Availability Content”; other statuses may take longer.
  • Storage: -20°C for 1 year
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at −20°C as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Mfr No BT-MCA0162
Alternative Names JUN; Transcription factor AP-1; Activator protein 1; AP1; Proto-oncogene c-Jun; V-jun avian sarcoma virus 17 oncogene homolog; p39
Clonality
  • Monoclonal
Concentration 1 mg/ml
Formulation Ascitic fluid containing 0.03% sodium azide,0.5% BSA, 50%glycerol.
Gene ID Human: 3725; Mouse: 16476
Host Mouse
Immunogen Purified recombinant fragment of human AP-1 expressed in E. Coli.
Isotype
  • IgG
Product Type
  • Primary Antibody
Reactivity
  • Human
  • Mouse
  • Monkey
Storage -20°C for 1 year

Product Overview

Research Use Only

This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.

Validated Applications

Western Blot (WB) See datasheet for recommended dilution
Immunohistochemistry (IHC) See datasheet for recommended dilution
Immunofluorescence (IF/ICC) See datasheet for recommended dilution
Flow Cytometry (FC) See datasheet for recommended dilution
ELISA (ELISA) See datasheet for recommended dilution

Key Specifications

Clonality Monoclonal
Host Species Mouse
Species Reactivity Human, Mouse, Monkey
Isotype IgG
Purification Affinity purification
Concentration 1 mg/ml
Formulation Ascitic fluid containing 0.03% sodium azide,0.5% BSA, 50%glycerol.
Catalog No. BT-MCA0162
UniProt Human: P05412; Mouse: P05627
Alternative Names JUN; Transcription factor AP-1; Activator protein 1; AP1; Proto-oncogene c-Jun; V-jun avian sarcoma virus 17 oncogene homolog; p39

Immunogen / Antigen

Purified recombinant fragment of human AP-1 expressed in E. Coli.

Reactivity Notes

This antibody has been validated for reactivity against Human, Mouse, Monkey targets. Cross-reactivity with other species may exist but has not been formally established.

Storage & Handling

-20°C for 1 year

Safety

For Research Use Only (RUO). Not intended for diagnostic, therapeutic, or veterinary use. Handle according to standard laboratory biosafety guidelines. Avoid skin and eye contact. Dispose of waste in accordance with local regulations.

Can antibody recognize the same target in different species from the datasheet? Has any other species been tested?

All of antibodies are generated with peptides designed using the database corresponding to the primary species listed in the datasheet. Generally they are human, mouse or rat. This species was then confirmed with the western blot validation data shown for each antibody in the appropriate species. If we do not obtain positive results in one species, we do not list that species. But the cell line we test possibly contains a low level of particular protein or the inductions we used do not work in that particular cell line. We suggest you compare the peptide sequence between the species you are studying and the species that BT Lab's antibody was raised against. In most cases, BT Lab's antibodies are raised against the human protein sequence and are highly purified using affinity chromatography.

Can BT Lab’s antibody be used in other application (WB, ELISA, IHC, IF, IP) which is not mentioned in the datasheet?

The instruction specifies the intended use for BT Lab's antibody. Any procedure not specified in the instruction should be validated by user.

What is the purity of BT Lab’s antibody?

Generally BT Lab offers antibodies with a purity higher than 96%.

How should I store my antibodies?

Please store the antibody at -20°C.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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