| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human APH1A was used as the immunogen for the APH1A antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
APH1A Antibody / Gamma-secretase subunit APH-1A is a anti-APH1A Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Flow cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB) with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm (ER, Golgi).
Key elements and design rationale
- Target: APH1A
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): FACS, IF, IHC, WB
Biological background
Functionally, APH1A antibody identifies a 265-amino-acid protein that serves as a structural scaffold for the gamma-secretase complex. APH1A forms stable interactions with Presenilin (PSEN1 or PSEN2), Nicastrin (NCSTN), and PEN2, facilitating proper folding, assembly, and trafficking of the complex. This assembly is required for the intramembrane proteolytic processing of substrates like APP, which produces amyloid-beta peptides linked to Alzheimer's disease pathogenesis. APH1A is also essential for Notch receptor cleavage, influencing cell fate determination and tissue differentiation.
The APH1A gene is located on chromosome 1q21.2 and encodes a protein localized to the endoplasmic reticulum and Golgi apparatus before its incorporation into the mature gamma-secretase complex at the plasma membrane. It contains seven transmembrane helices and conserved motifs necessary for Presenilin binding and enzymatic activity. APH1A is one of two APH1 homologs in humans, the other being APH1B, which participates in distinct gamma-secretase isoforms with varying substrate preferences and tissue expression patterns.
Mutations or dysregulation of APH1A disrupt gamma-secretase assembly and reduce proteolytic activity, leading to defects in Notch signaling and accumulation of unprocessed APP. These abnormalities contribute to neurodevelopmental disorders and neurodegenerative diseases, including Alzheimer's disease. Experimental studies show that selective regulation of APH1A-containing gamma-secretase complexes can modulate amyloid-beta production without affecting Notch cleavage, providing a potential therapeutic strategy.
APH1A antibody is widely used in neurobiology, signal transduction, and protein trafficking research. It is suitable for immunoblotting, immunohistochemistry, and co-immunoprecipitation to examine gamma-secretase composition and function. In Alzheimer's research, APH1A detection helps analyze isoform-specific gamma-secretase activity and its modulation by small molecules or genetic factors.
Structurally, APH1A acts as a transmembrane scaffold stabilizing the Presenilin catalytic core and maintaining the architecture of the protease complex. Its interactions are regulated by lipid environment and post-translational modifications such as glycosylation.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
