| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human APOBEC3C was used as the immunogen for the APOBEC3C antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
APOBEC3C Antibody is a anti-APOBEC3C Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear, cytoplasmic.
Key elements and design rationale
- Target: APOBEC3C
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC, IF, FACS
Biological background
Structurally, APOBEC3C contains a single catalytic zinc-dependent deaminase domain characterized by the H-X-E-X(23-28)-P-C-X2-C motif, which coordinates zinc ions for catalytic activity. It primarily targets cytosines within the 5'-TC context of viral DNA, leading to G-to-A hypermutations that inhibit viral genome integrity. APOBEC3C acts against human immunodeficiency virus type 1 (HIV-1) and other retroelements by editing viral cDNA during replication. Its antiviral activity is antagonized by viral infectivity factor (Vif) proteins that mediate APOBEC degradation via the ubiquitin-proteasome pathway.
The APOBEC3C antibody is used in virology, immunology, and genomic stability research to study intrinsic antiviral immunity and cytidine deamination mechanisms. Western blot analysis detects a 23 kilodalton band corresponding to APOBEC3C, while immunofluorescence shows cytoplasmic and nuclear localization depending on infection or stress conditions. This antibody supports the investigation of APOBEC3C-mediated mutagenesis, viral restriction, and immune regulation.
Beyond antiviral defense, APOBEC3C influences genomic diversity and cancer mutagenesis by contributing to localized DNA editing events. Dysregulation or overexpression of APOBEC family enzymes, including APOBEC3C, has been linked to mutational signatures in various cancers. The APOBEC3C antibody provides a reliable tool for exploring the dual roles of APOBEC3C in immunity and genome evolution.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
