| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | Recombinant human protein (amino acids L7-S698) was used as the immunogen for the AqCbl reductase antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
AqCbl reductase Antibody / Aquacobalamin reductase / MTRR is a anti-MTRR Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), IHC-P, Immunofluorescence (IF), Direct ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm.
Key elements and design rationale
- Target: MTRR
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Antigen affinity purified
- Applications (as listed): WB, FACS, IHC-P, IF, Direct ELISA
Biological background
Dysregulation of AqCbl reductase activity can disrupt cobalamin homeostasis and is associated with metabolic disorders, including methylmalonic acidemia and homocystinuria. Given its pivotal role in cobalamin processing, AqCbl reductase has emerged as a critical marker in studies of vitamin B12 metabolism and related diseases. Researchers have also explored its function in oxidative stress responses and mitochondrial health.
The AqCbl reductase antibody enables specific detection of this enzyme in biological samples, supporting studies of cobalamin-dependent metabolic pathways. It is widely used in western blot, immunohistochemistry, and ELISA applications to investigate tissue-specific expression patterns and disease-related changes. With proven performance and high specificity, the AqCbl reductase antibody is an indispensable tool for scientists exploring vitamin B12 metabolism. Incorporating the AqCbl reductase antibody into your research workflow provides valuable insights into the regulation and function of this essential metabolic enzyme.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
