| Field | Specification |
|---|---|
| Product Type | |
| Shipping | |
| Source | Recombinant (E. coli) |
| Storage |
ArCas12a, derived from the CRISPR system of Agathobacter rectalis bacteria and also known as Cpf1, is a monomeric protein consisting of 1263 amino acids. As a class II (type V) member of the CRISPR/Cas system, ArCas12a functions solely through a single effector protein and exhibits significant differences from Cas9, such as targeting motifs rich in T, not requiring trans-activating crRNA, producing sticky ends upon DNA double-strand breaks, participating in RNA processing, and possessing DNA nuclease activity.
ArCas12a lacks the HNH domain and can independently utilize its RuvC domain to recognize the PAM region rich in thymine (T) at the 5' end of the target nucleic acid under the guidance of CRISPR RNA (crRNA), initiating cleavage of the target DNA. It has been successfully used for genome editing in many mammals and plants. Additionally, ArCas12a exhibits trans-cleavage activity, capable of indiscriminately cutting non-target single-stranded DNA (ssDNA) in the reaction system.
Compared to other LbCas12a/AsCas12a, ArCas12a demonstrates higher temperature adaptability (25-55oC), making it suitable for genome editing and nucleic acid detection applications.
Features
Broad reaction temperature range: Exhibits cleavage activity within the temperature range of 25-55oC
Low nuclease residue: No residual exonuclease, nickase, or RNase
Cis-cleavage activity: Highly Effective Cleavage of Double-Stranded DNA in Vitro
Trans-cleavage activity: High trans-cleavage activity, suitable for nucleic acid detection
Applications
CRISPR/Cas gene editing
Diagnostic and detection based on the CRISPR/Cas system
Other detection applications combined with isothermal nucleic acid amplification technologies (RPA and LAMP), etc
Specifications
|
Source |
The Cpf1 gene from Agathobacter rectalis is expressed through recombinant expression in E.coli |
|
Molecular weight |
149 KDa |
|
PAM sequence |
TTTN or TTTV |
|
Reaction conditions |
50 mM NaCl, 10 mM Tris-HCl, 10 mM MgCl2, pH 7.9 @25oC |
|
Concentration |
10 µM |
|
Purity |
>95% (SDS-PAGE) |
|
Inactivation conditions |
85℃, 5-10 min |
Glycerol Content |
Contains Glycerol |
Components
|
Components No. |
Name |
14702ES65 |
14702ES80 |
|
14702-A |
ArCas12a Nuclease (10 μM) |
100 μL |
|
|
14702-B |
10×ArCas12a Reaction Buffer |
1 mL |
1 mL |
Shipping and Storage
This product should be stored at -25 ~ -15oC for 2 years.
Figures
Figure 1. Test of ArCas12a cis-cleavage activity: M: Marker; C: Template double-stranded DNA (dsDNA)
Note: Under the guidance of crRNA, it can efficiently cleave dsDNA (600 bp) to produce two fragments (200 bp + 400 bp).
Figure 2. ArCas12a Trans-cleavage Activity Test Results
Note: Using dsDNA as the target, ArCas12a, crRNA, and a ssDNA reporter probe (containing a fluorescent reporter group) were added for in vitro cleavage. Once ArCas12a forms a complex with crRNA and the target DNA, it activates trans-cleavage activity, cutting the ssDNA reporter probe, thereby emitting fluorescence.
Store this enzyme at -20°C and avoid repeated freeze-thaw cycles to preserve catalytic activity. The product is shipped Dry Ice and remains stable for up to one year from the date of manufacture when stored under recommended conditions. Aliquoting the stock solution into single-use volumes is recommended for enzymes used infrequently to minimize thermal cycling of the bulk stock.
CRISPR/Cas gene editing Diagnostic and detection based on the CRISPR/Cas system Other detection applications combined with isothermal nucleic acid amplification technologies (RPA and LAMP), etc. Always verify compatibility with your specific template, buffer, and downstream workflow.
Nuclease activity is expressed in µg/µL; cleavage efficiency is validated as the percentage of target DNA cut within 60 min at 37°C using a defined guide RNA and target plasmid substrate.
This enzyme is produced as Recombinant (E. coli) and supplied as a Research Use Only (RUO) reagent. Each lot is subjected to activity assay, purity assessment by SDS-PAGE, and functional validation prior to release. A Certificate of Analysis (CoA) and Safety Data Sheet (SDS) are available on request.
On-target efficiency is primarily determined by guide RNA (gRNA) design, target accessibility within chromatin, and PAM sequence context. Off-target cleavage is influenced by sequence complementarity mismatches (particularly in the seed region proximal to the PAM), enzyme concentration, and delivery method. High-fidelity Cas9 variants with engineered positional charge substitutions significantly reduce off-target activity (>99% reduction) while maintaining on-target cleavage efficiency comparable to wild-type enzyme.
Yeasen Biotechnology supports custom enzyme solutions across multiple service lines — from GMP-grade bulk supply to directed enzyme engineering. Contact BioHippo to discuss requirements and initiate a project inquiry.
▶ GMP-Grade & Bulk Supply
Select Yeasen enzymes are available in GMP grade, manufactured in an ISO 13485-certified UCF.ME™ ultra-clean molecular enzyme facility with FDA Drug Master File (DMF) support.
- GMP-grade release testing and CoA documentation
- ISO 13485-certified production facility
- Scalable from milligram to multi-gram quantities
- Consistent lot-to-lot activity specifications
▶ Glycerol-Free & Custom Formulation
Glycerol-free enzyme formats are available for applications requiring lyophilization compatibility, liquid handling automation, or direct IVD master mix integration.
- Glycerol-free liquid format (standard and custom buffers)
- Lyophilization-ready enzyme preparation
- Custom reaction buffer optimization for specific assay conditions
- Compatible with freeze-drying workflows for point-of-care formats
▶ Molecular IVD RDC Service
Yeasen's Research and Development Contracting (RDC) team delivers end-to-end solutions for molecular diagnostic product development, covering enzyme selection through clinical validation support.
- Enzyme selection and performance matching
- Primer/probe design and reaction buffer optimization
- Sensitivity, specificity, and precision validation studies
- Stability studies and SNP evaluation
- Instrument platform compatibility assessment
▶ ZymeEditor™ Enzyme Engineering
Yeasen's proprietary ZymeEditor™ directed evolution and rational design platform enables the development of custom enzyme variants with tailored performance characteristics not available in off-the-shelf products.
- Directed evolution for enhanced thermostability, processivity, or fidelity
- Rational design for altered substrate specificity or cofactor requirements
- Library screening from Yeasen's proprietary enzyme variant collection
- Scale-up to commercial quantities upon candidate confirmation
ⓘ Customization services are fulfilled by Yeasen Biotechnology. Lead times and minimum order quantities vary by service type. Contact BioHippo for project scoping and pricing.
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