| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Product Type | |
| Promoter | |
| Reporter | |
| Selection Marker | Blasticidin, N/A, Puromycin |
| Shipping | |
| Species |
Background
The antioxidant response element (ARE) is a cis-regulatory DNA sequence found in the promoters of genes encoding detoxification and antioxidant enzymes. It is the principal binding site for NFE2L2 (NRF2) and the related factor NFE2L1 (NRF1), basic leucine zipper transcription factors that orchestrate the cellular defense against oxidative and electrophilic stress. Under basal conditions NRF2 is held in the cytoplasm by KEAP1 and targeted for degradation; oxidative stress stabilizes NRF2, allowing it to enter the nucleus, dimerize with small MAF proteins, and activate ARE-driven transcription. This cytoprotective program is central to redox homeostasis, and its dysregulation contributes to inflammation, neurodegeneration, and cancer.
Product Description & Applications
The ARE Reporter Lentivirus is a lentiviral reporter system for monitoring NRF1/NRF2 antioxidant signaling in human or mouse cells. An antioxidant response element placed upstream of a fluorescent or luminescent reporter (GFP, RFP, firefly luciferase, with Renilla luciferase available) provides a sensitive readout of NRF1/2 transcriptional activity, enabling detection of antioxidant pathway activation in transduced cells. Optional antibiotic selection markers (puromycin or blasticidin) support generation of stable reporter cell lines. The product is well suited to studying oxidative stress responses, screening NRF2 activators or inhibitors, and characterizing redox signaling. Supplied as lentiviral particles purified by PEG precipitation and sucrose gradient centrifugation, it efficiently transduces difficult-to-transfect cells, including primary and thawed cells.
About This Product
This reporter lentivirus places a Firefly Luc, GFP, Luc, Renilla Luc, RFP reporter gene under the control of tandem consensus response elements specific for the NRF1/2 antioxidant pathway transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
