| Field | Specification |
|---|---|
| Activity | |
| Biological Activity | |
| Enzyme Type | |
| Expression System | |
| Form | Liquid |
| Formulation | |
| Product Type | |
| Purity | |
| Source | Escherichia Coli. |
| Species | |
| Storage | |
| Target |
Product Description
Arginase-1, Active Human Recombinant
Overview
Arginase-1 is part of the urea cycle, it catalyzes the hydrolysis of arginine to ornithine and urea. There are two isoforms of mammalian arginase which differ in their tissue location, subcellular localization, immunologic crossreactivity & physiologic role. Arginase-1is a cytosolic enzyme and expressed primarily in the liver tissue. Inherited deficiency in this enzyme may lead toargininemia, which is an autosomal recessive disease in which hyperammonemia is detected.
Biological Activity
Specific activity is > 150,000 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of arginine to urea per minute at pH 10.5 at 37C.
Specifications
| Purity | Greater than 85.0% as determined by SDS-PAGE. |
|---|---|
| Formulation | ARG1 protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 2mM DTT and 100mM NaCl. |
| Storage | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Expression System | Escherichia Coli. |
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