| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human GEF-H1/ARHGEF2 recombinant protein (Position: Q94-E923) was used as the immunogen for the ARHGEF2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
ARHGEF2 Antibody / Rho guanine nucleotide exchange factor 2 / GEF-H1 is a anti-ARHGEF2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm.
Key elements and design rationale
- Target: ARHGEF2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, FACS, ELISA
Biological background
Functionally, ARHGEF2 antibody identifies a 986-amino-acid cytoplasmic protein containing Dbl homology (DH) and pleckstrin homology (PH) domains responsible for its guanine nucleotide exchange activity. ARHGEF2 activates RHOA and RHOC, stimulating actin stress fiber formation, contractility, and focal adhesion turnover. When bound to microtubules, ARHGEF2 remains inactive; upon microtubule depolymerization, it is released into the cytoplasm, where it activates RHOA signaling.
The ARHGEF2 gene is located on chromosome 1q22 and encodes a protein expressed in epithelial cells, neurons, and endothelial tissues. ARHGEF2 plays a key role in linking microtubule dynamics to actin cytoskeletal remodeling, influencing cell junction integrity and migration. In epithelial cells, it regulates tight junction assembly and polarity through interactions with PAR6 and AMPK. In neurons, ARHGEF2 contributes to axon guidance and dendritic spine morphology.
Pathologically, dysregulation of ARHGEF2 activity has been associated with cancer progression, inflammation, and neurodevelopmental disorders. Overactivation of RHOA signaling by ARHGEF2 promotes tumor invasion, while loss of its regulation can impair barrier function in inflammatory bowel disease. ARHGEF2 also mediates cytokine-induced cytoskeletal remodeling during immune responses, linking microtubule organization to inflammatory signaling pathways.
ARHGEF2 antibody is widely used in cytoskeletal, cell signaling, and cancer biology research. It is suitable for immunoblotting, immunofluorescence, and immunoprecipitation to detect ARHGEF2 expression and RHOA activation states. This antibody supports studies of cell polarity, microtubule-actin crosstalk, and epithelial integrity. In biomedical research, ARHGEF2 serves as a molecular bridge between cytoskeletal regulation and signal transduction.
Structurally, ARHGEF2 contains modular domains for RHOA interaction, microtubule binding, and regulation by phosphorylation. Its activity is modulated by kinases including MARK2 and Aurora A, which control its release from microtubules.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
