| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human ARHGEF6 recombinant protein (Position: E54-R765) was used as the immunogen for the ARHGEF6 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
ARHGEF6 Antibody / Rho guanine nucleotide exchange factor 6 is a anti-ARHGEF6 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse.
Key elements and design rationale
- Target: ARHGEF6
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
Functionally, ARHGEF6 antibody identifies a 776-amino-acid scaffold protein containing SH3, DH, and PH domains that mediate interaction with PAK kinases and other signaling adaptors. ARHGEF6 promotes actin cytoskeleton rearrangements, lamellipodia formation, and cell adhesion dynamics through activation of Rho GTPase signaling cascades.
The ARHGEF6 gene is located on chromosome Xq26.3 and is highly expressed in the brain, immune cells, and epithelial tissues. It participates in synaptic development, immune cell migration, and epithelial junction organization by coupling surface receptors to cytoskeletal effectors.
Pathologically, mutations in ARHGEF6 cause X-linked intellectual disability due to impaired synaptic signaling and dendritic spine morphology. Aberrant ARHGEF6 expression is also associated with immune dysfunction and metastatic cancer cell migration. Research using ARHGEF6 antibody supports studies in cell signaling, cytoskeletal regulation, and neurological disorders.
ARHGEF6 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect guanine nucleotide exchange factors.
Structurally, Rho guanine nucleotide exchange factor 6 forms multiprotein complexes with PAK kinases and GIT proteins, linking receptor activation to localized actin assembly. This antibody enables exploration of ARHGEF6's molecular function in Rho GTPase activation and cellular morphogenesis.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
