| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human ARHGEF7 recombinant protein (Position: S25-N637) was used as the immunogen for the ARHGEF7 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
ARHGEF7 Antibody / Beta-Pix / Rho guanine nucleotide exchange factor 7 is a anti-ARHGEF7 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm.
Key elements and design rationale
- Target: ARHGEF7
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC, IF, FACS, ELISA
Biological background
Structurally, ARHGEF7 contains several modular domains, including an SH3 domain that interacts with PAK kinases, a DH (Dbl homology) domain responsible for nucleotide exchange activity, and a PH (pleckstrin homology) domain that binds phosphoinositides for membrane targeting. These domains enable ARHGEF7 to act as a scaffold connecting small GTPases, kinases, and adhesion proteins such as GIT1 and paxillin. It localizes predominantly at focal adhesions and synaptic sites, integrating extracellular signals with intracellular cytoskeletal responses.
The ARHGEF7 antibody is widely used in cell signaling, cancer biology, and neurobiology research to study cytoskeletal organization, cell motility, and synaptic regulation. Western blot analysis detects a 76 kilodalton band corresponding to ARHGEF7, while immunofluorescence reveals punctate staining along the cell cortex, focal adhesions, and neuronal synapses. This antibody supports exploration of actin dynamics and Rho GTPase signaling in both normal and pathological contexts.
Functionally, ARHGEF7 interacts with p21-activated kinases (PAKs) to form complexes that control Rac1/Cdc42 activation and downstream signaling to the MAPK and JNK pathways. In neurons, it contributes to dendritic spine formation and synaptic plasticity by linking postsynaptic density proteins to actin assembly machinery. Dysregulation of ARHGEF7 has been associated with cancer metastasis, psychiatric disorders, and developmental defects due to altered Rho signaling. The ARHGEF7 antibody provides a dependable tool for dissecting these pathways and quantifying GEF expression in various models.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
