| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human ARID3A was used as the immunogen for the ARID3A antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
ARID3A Antibody / AT-rich interactive domain-containing protein 3A is a anti-ARID3A Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Flow cytometry (FACS), Immunoprecipitation (IP), Western blot (WB) with listed reactivity in Human.
Key elements and design rationale
- Target: ARID3A
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): FACS, IP, WB
Biological background
AT-rich interactive domain-containing protein 3A plays an essential role in B-cell development and immunoglobulin gene transcription. It promotes accessibility of heavy-chain enhancer regions and is crucial for the proper differentiation of pre-B and mature B lymphocytes. The ARID3A antibody is widely used in immunology and developmental biology research to assess how ARID3A expression correlates with cell fate and lineage commitment. Alterations in its expression have been associated with autoimmune disorders, hematological malignancies, and aberrant immune signaling pathways.
Beyond hematopoiesis, ARID3A contributes to embryonic patterning and organogenesis. It interacts with other chromatin regulators to influence developmental gene programs in neural and epithelial cells. Dysregulated expression can result in abnormal proliferation, reduced differentiation, and oncogenic transformation. The ARID3A antibody allows for high-resolution detection of this transcription factor in tissue and cell-based assays, helping define how ARID3A participates in chromatin remodeling, enhancer activation, and epigenetic memory.
In experimental use, the ARID3A antibody performs effectively in western blotting, immunofluorescence, chromatin immunoprecipitation, and immunohistochemistry. These applications reveal protein abundance, nuclear localization, and DNA-binding site occupancy. Through combination with transcriptomic analysis, the ARID3A antibody supports integrative studies of gene regulation, highlighting how AT-rich interactive domain-containing protein 3A interacts with co-factors to orchestrate transcriptional networks governing development and immunity.
ARID3A expression patterns are also linked to tumor progression and metastasis. Elevated or suppressed levels of the protein can influence tumor suppressor pathways and DNA repair mechanisms. The ARID3A antibody from
By facilitating the study of AT-rich interactive domain-containing protein 3A, the ARID3A antibody continues to advance molecular understanding of chromatin organization and gene regulation. Its value extends from developmental and immunological research to clinical oncology, where deciphering ARID3A function may lead to improved diagnostics and targeted therapies. The antibody's consistent performance makes it an essential reagent for decoding the transcriptional architecture underlying human biology and disease.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
