| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human ARL2 was used as the immunogen for the ARL2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
ARL2 Antibody / ADP-ribosylation factor-like protein 2 is a anti-ARL2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasmic, Nuclear.
Key elements and design rationale
- Target: ARL2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC, IF, FACS
Biological background
Structurally, ARL2 is a 184-amino-acid cytosolic protein of approximately 21 kilodaltons containing the characteristic GTP-binding motifs (G1-G5 boxes) required for nucleotide exchange and hydrolysis. It interacts with cofactors such as cofactor D (TBCD) and cofactor E (TBCE) to modulate tubulin folding and polymerization. ARL2 localizes to both cytoplasm and mitochondria, where it forms complexes that support microtubule stability and mitochondrial fusion.
The ARL2 antibody is widely used in cell biology, neurobiology, and mitochondrial research to study small GTPase signaling, cytoskeletal organization, and intracellular trafficking. Western blot analysis detects a 21 kilodalton band corresponding to ARL2, while immunofluorescence demonstrates cytoplasmic and mitochondrial staining patterns. This antibody provides a dependable tool for monitoring ARL2-mediated control of tubulin dynamics and organelle function.
Functionally, ARL2 regulates the folding of alpha and beta tubulin heterodimers, supporting microtubule assembly critical for intracellular transport and cell division. Within mitochondria, ARL2 influences fusion, morphology, and ATP production by interacting with proteins such as ANT1 and ELMOD2. Dysregulation of ARL2 expression affects mitochondrial integrity, axonal transport, and cell viability. In neurons, ARL2 contributes to microtubule-based trafficking of synaptic vesicles and organelles. The ARL2 antibody enables analysis of these processes and provides insights into cytoskeletal and metabolic coordination.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
