| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human ARL3 was used as the immunogen for the ARL3 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
ARL3 Antibody / ADP-ribosylation factor-like protein 3 is a anti-ARL3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear, cytoplasmic.
Key elements and design rationale
- Target: ARL3
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC, IF, FACS
Biological background
ADP-ribosylation factor-like protein 3 cycles between GTP- and GDP-bound states, regulating the release of lipidated cargo from carrier proteins such as PDE6D and UNC119. The ARL3 antibody supports studies that monitor these molecular interactions and visualize ARL3 localization within cilia, photoreceptor cells, and centrosomes. Its activation is mediated by the guanine nucleotide exchange factor ARL13B, while GTP hydrolysis is stimulated by the GTPase-activating protein RP2. Together, these interactions ensure precise spatiotemporal control of cargo delivery.
Mutations in the ARL3 gene cause Joubert syndrome and cone-rod dystrophy, disorders associated with ciliary dysfunction and photoreceptor degeneration. The ARL3 antibody is critical for studying these ciliopathies, enabling detection of expression changes and subcellular mislocalization of mutant proteins. Disruption of ARL3-mediated trafficking leads to accumulation of mislocalized proteins and progressive loss of photoreceptor function.
Beyond the retina, ADP-ribosylation factor-like protein 3 regulates microtubule organization and vesicle transport in other ciliated and non-ciliated cells. The ARL3 antibody supports investigations into these broader cellular functions, including roles in signal transduction and organelle positioning. ARL3 also contributes to lipid modification cycles that control the targeting of myristoylated and prenylated proteins.
The ARL3 antibody performs effectively in western blotting, immunofluorescence, and immunohistochemistry, displaying distinct ciliary and pericentrosomal staining consistent with its trafficking role.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
