| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human ASCL1 was used as the immunogen for the ASCL1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
ASCL1 Antibody / Achaete-scute homolog 1 is a anti-ASCL1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear, cytoplasmic.
Key elements and design rationale
- Target: ASCL1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, FACS
Biological background
ASCL1 is encoded by the ASCL1 gene on human chromosome 12q23.2. The protein is approximately 194 amino acids long and contains a highly conserved bHLH domain that mediates DNA binding and dimerization with E proteins. ASCL1 recognizes E-box motifs within target gene promoters to activate transcription of neuronal genes, including those involved in axon guidance, neurotransmitter synthesis, and synaptic formation.
The ASCL1 antibody detects a 45 kilodalton protein by western blot and exhibits nuclear staining in differentiating neuronal populations. ASCL1 expression peaks during early neurogenesis and declines as cells mature into neurons. It functions in concert with other transcription factors such as NEUROD1 and POU3F2 to coordinate the transition from proliferating progenitors to postmitotic neurons.
In addition to its developmental role, ASCL1 is reactivated in neuroendocrine tumors such as small-cell lung carcinoma, where it drives expression of genes promoting proliferation and neuroendocrine identity. ASCL1 also serves as a key transcriptional reprogramming factor used to convert fibroblasts into induced neurons in vitro, illustrating its capacity to initiate neuronal gene networks in non-neural cells.
Because ASCL1 integrates differentiation and proliferation control, it serves as a critical model for studying lineage specification and transcriptional reprogramming.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
