| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A portion of amino acids 38-65 from the human protein was used as the immunogen for this ASCL2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
ASCL2 Antibody / Achaete-scute homolog 2 is an antibody targeting ASCL2, raised in Rabbit for protein detection and localization studies where these specifications are required.
Key elements and design rationale
- Target: ASCL2.
- Antibody identity: Polyclonal (rabbit origin); Rabbit Ig.
- Conjugate/label: Unconjugated (affects detection chemistry and multiplex compatibility).
- Format: Antigen affinity purified.
- Species reactivity: Human, Mouse.
- Listed applications: WB (refer to on-page specifications for application-specific guidance).
Biological background
ASCL2 belongs to a family of genes that are involved in determining cell fate and differentiation, particularly in the development of tissues such as the nervous system and the intestines. Studies have shown that ASCL2 plays a critical role in regulating the self-renewal and differentiation of stem cells, making it a key player in tissue regeneration and repair processes. One of the most fascinating aspects of ASCL2 is its ability to control the expression of other genes, essentially acting as a master regulator in the cellular development process. By activating or repressing specific genes, ASCL2 can influence the fate of a cell and dictate its ultimate function within a tissue or organ. ASCL2 has been implicated in a variety of diseases and conditions, including certain types of cancers where its dysregulation can lead to uncontrolled cell growth and proliferation.
Research relevance and current trends
- Comparative expression profiling across cell types, tissues, or perturbations (e.g., drug treatment, genetic editing, or differentiation).
- Subcellular localization and trafficking studies, including co-localization with pathway markers in microscopy-based assays.
- Integration of protein-level measurements with transcriptomics or proteomics to relate abundance to regulation and phenotype.
Common research applications
- Western blotting: researchers commonly compare relative signal levels across conditions and use appropriate negative/positive controls for interpretation.
Interpretation should account for antibody-dependent factors such as epitope accessibility, isoforms, and sample preparation differences across workflows.
Notes for experimental interpretation
- Isoforms and PTMs: many targets have multiple isoforms and post-translational modifications that can shift apparent signal or localization; interpret bands/signals accordingly.
- Epitope context: binding can depend on protein conformation and sample processing; region information in the title/immunogen can help anticipate what may be detected.
- Species differences: predicted or validated reactivity may vary by ortholog sequence and sample context; confirm in your model system.
- Control concepts: include negative controls (no-primary/isotype), and where possible genetic controls (KO/KD) or independent antibodies to strengthen conclusions.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
