| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | Recombinant human ASRGL1 fragment from the human protein was used as the immunogen for the ASRGL1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
ASRGL1 (Asparaginase-like protein 1), also known as CRASH, is a 308 amino acid protein belonging to the Ntn-hydrolase family. Asparaginases utilize asparagine as a substrate to produce aspartic acid and ammonia. ASRGL1 has been identified as a autoantigenic protein that is present in the mid-piece of sperm after obstruction of the male reproductive tract. ASRGL1 is expressed highly in testis, but is also expressed in brain, kidney and gastrointestinal tissues. High levels of ASRGL1 have also been identified in ovarian, uterine and mammary tumors in comparison with normal tissues of the same origin.
This anti-ALP1 antibody is supplied as Purified (Mouse, Monoclonal (mouse origin), clone CRASH/1290, Mouse IgG1, kappa, Unconjugated) and is designed to support common target-detection workflows after the on-page specifications.
Key elements and design rationale
- Target: ALP1
- Format: Purified
- Localization: Cytoplasmic, nuclear
- Species reactivity: Human
- Applications (listed): ELISA
- Conjugate: Unconjugated
- Clone and antibody class: Monoclonal (mouse origin), clone CRASH/1290, Mouse IgG1, kappa
Because antibody performance can depend on epitope context, sample preparation, and biological state, interpret signals using appropriate controls and orthogonal evidence when possible.
Biological background
ALP1 is referenced in public gene/protein resources (e.g., UniProt and NCBI Gene), which provide curated names/synonyms, protein features, and pathway context. When designing assays, consider potential isoforms, post-translational modifications, and cell-type specific expression that may influence observed signal.
Research relevance and current trends
- Profiling ALP1 expression across model systems, perturbations, and time points to support mechanistic hypotheses.
- Combining antibody-based detection with multi-omics or imaging readouts to link ALP1 signal with phenotype.
- Using well-matched controls (isotype controls, genetic perturbations, or independent reagents) to strengthen interpretation of target-associated signal.
Common research applications
- ELISA
Use the listed applications as a starting point and tailor experimental design to your sample type and readout requirements.
Notes for experimental interpretation
- Specificity considerations: closely related family members, isoforms, or PTMs can affect apparent specificity; confirm with independent approaches when critical.
- Controls: include negative controls and, when feasible, genetic or pharmacologic perturbations to support target attribution in your system.
- Species and sample context: differences in sequence, expression, fixation, or extraction conditions can change signal behavior across models.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
