| Field | Specification |
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| Host | |
| Immunogen | A synthesized peptide derived from human ATE1 was used as the immunogen for the ATE1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
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| Target | |
| UniProt # |
Overview
ATE1 Antibody / Arginyl transferase 1 is a anti-ATE1 Rabbit antibody Recombinant Rabbit Monoclonal clone 30A62 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: ATE1
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 30A62, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC
Biological background
ATE1 antibody is widely applied in protein modification research, developmental biology, and cell signaling. Arginylation is part of the N end rule pathway of protein degradation, where the identity of the N terminal residue determines protein half life. By detecting ATE1, researchers can study how this enzyme contributes to protein turnover, quality control, and cellular adaptation.
Western blotting with ATE1 antibody detects enzyme expression in heart, liver, and brain. Immunohistochemistry maps ATE1 in tissues undergoing development or stress, while immunofluorescence reveals cytoplasmic localization and associations with cytoskeletal structures. These applications provide valuable tools for understanding protein arginylation across biological systems.
ATE1 plays a critical role in cardiovascular biology. Knockout studies in mice demonstrate that ATE1 is essential for heart development and angiogenesis. Loss of arginylation disrupts actin filament organization and cellular migration. By applying ATE1 antibody, scientists can explore how arginylation shapes cardiovascular and developmental pathways.
In cell biology, ATE1 regulates actin and other cytoskeletal proteins, modulating cell adhesion and motility. Arginylation also affects metabolic enzymes and stress response proteins, linking ATE1 to adaptation under hypoxia or oxidative stress. Dysregulation of ATE1 has been associated with cancer progression, where altered protein stability influences tumor growth and metastasis. The antibody thus supports both basic and translational research.
ATE1 also contributes to neurological development and function. Arginylation of synaptic proteins influences plasticity and neuronal survival. This expands the relevance of ATE1 antibody to studies in neurobiology and neurodegenerative disease. Ongoing research highlights ATE1 as a potential therapeutic target for modulating protein stability and cellular adaptation.
ATE1 antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
