| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human ATF5 recombinant protein (Position: L11-R275) was used as the immunogen for the ATF5 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
ATF5 Antibody / Activating transcription factor 5 is a anti-ATF5 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: ATF5
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
Structurally, ATF5 is a 282-amino-acid nuclear protein of approximately 33 kilodaltons containing a basic leucine zipper (bZIP) domain that mediates DNA binding and dimerization with other bZIP family members. Through its N-terminal transactivation domain, ATF5 regulates gene expression involved in apoptosis suppression, differentiation control, and autophagy induction. It is ubiquitously expressed but shows high abundance in neural tissues, liver, and testis, where it participates in differentiation and stress adaptation.
The ATF5 antibody is widely used in neuroscience, oncology, and stress physiology research to study transcriptional regulation and survival signaling. Western blot analysis detects a 33 kilodalton band corresponding to ATF5, while immunofluorescence reveals strong nuclear localization and induction under stress conditions. This antibody enables researchers to analyze transcriptional programs governing neurogenesis, stress adaptation, and tumor resistance.
Functionally, ATF5 promotes cell survival under stress by inducing anti-apoptotic genes such as BCL2 and HSPA5 while repressing pro-apoptotic transcriptional regulators. In neurons, ATF5 regulates differentiation of neural progenitors and glial cells, influencing brain development and repair. In cancer cells, ATF5 expression confers resistance to nutrient and hypoxia-induced apoptosis, contributing to tumor growth and therapy resistance. The ATF5 antibody provides a reliable reagent for monitoring ATF5 activity in normal and stress-related transcriptional responses.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
