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CCK-8 uses WST-8 [2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium] as a colorimetric indicator of cellular dehydrogenase activity. Living cells reduce WST-8 to a soluble orange formazan product — the amount of formazan generated is directly proportional to the number of metabolically active (viable) cells. ATP Luminescent kits measure intracellular ATP as a proxy for cell viability via bioluminescence.
Standard CCK-8 and 2D ATP assays are optimized for monolayer cultures. The 3D ATP Luminescent Cell Viability Assay Kit is specifically formulated for 3D spheroid models, incorporating enhanced lysis and solubilization chemistry to penetrate compact spheroid cores and recover total ATP signal. For standard CCK-8 with 3D cultures, extended incubation or matrix dissolution may be required — consult the datasheet.
Yes, provided the final DMSO concentration in the assay does not exceed 0.1–0.5% (v/v). Higher DMSO concentrations can independently affect cell metabolism and formazan/ATP generation, confounding viability readout. Always include DMSO vehicle controls at matched concentrations and confirm DMSO tolerance for your specific cell type before running compound screens.
CCK-8: Absorbance measurement at 450 nm (reference wavelength 620–650 nm). ATP Luminescent assays: Luminescence mode (no excitation filter). Ensure the instrument is equilibrated to room temperature and warmed up before measurement. For kinetic assays using CCK-8, the plate can remain in the incubator between reads — the assay is non-lytic and allows repeated measurement.
Yeasen Biotechnology offers flexible customization options for many of its assay kits and detection reagents, including custom lot sizes, bulk ordering, and application-specific formulation adjustments. Volume pricing, custom packaging, and kit bundling may be available depending on the product and intended workflow. A Certificate of Analysis (CoA) and lot-specific QC data are provided with every order. For inquiries regarding large-volume orders, custom configurations, or integration into automated workflows, please contact the BioHippo team for a tailored quotation.
