| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human ATP5G1 was used as the immunogen for the ATP5G1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
ATP5G1 Antibody / ATP5MC1 is a anti-ATP5G1 Rabbit antibody Recombinant Rabbit Monoclonal clone 29A74 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: ATP5G1
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 29A74, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC
Biological background
ATP synthase consists of two major domains: the F1 catalytic domain, which synthesizes ATP from ADP and inorganic phosphate, and the F0 membrane domain, which translocates protons across the inner mitochondrial membrane. ATP5G1 is one of the small c subunits that form the proton conducting ring of the F0 domain. Research using ATP5G1 antibody has revealed that this protein ensures efficient proton transport and rotary motion, allowing the enzyme to generate ATP that fuels cellular metabolism. Disruption of this subunit destabilizes ATP synthase and reduces energy production.
Mutations in ATP5G1 and related subunits have been associated with mitochondrial diseases, including encephalomyopathy and metabolic syndromes. Studies with ATP5G1 antibody have confirmed that defects impair ATP production and contribute to developmental delay, neuromuscular symptoms, and metabolic acidosis. Because ATP synthase is fundamental for energy metabolism, ATP5G1 remains essential for tissue homeostasis in high energy demanding organs such as brain, muscle, and heart.
In addition to inherited disorders, mitochondrial dysfunction involving ATP synthase subunits contributes to aging, neurodegeneration, and cancer. Research using ATP5G1 antibody has demonstrated altered expression in tumors, where shifts in energy metabolism favor glycolysis over oxidative phosphorylation. In neurons, reduced ATP synthase activity contributes to synaptic dysfunction and neurodegeneration. These findings highlight the broad significance of ATP5G1 in health and disease.
ATP5G1 antibody is widely used in western blotting, immunohistochemistry, and immunofluorescence. Western blotting demonstrates abundance in mitochondria rich tissues, immunohistochemistry highlights distribution in heart, muscle, and brain, and immunofluorescence reveals localization to mitochondrial networks. Together, these methods establish ATP5G1 antibody as a core tool for mitochondrial research.
By providing validated ATP5G1 antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
