| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human ATP5G1 was used as the immunogen for the ATP5MC1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
ATP5G1 Antibody / ATP5MC1 is a anti-ATP5G1 Rabbit antibody Recombinant Rabbit Monoclonal clone 29A75 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: ATP5G1
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 29A75, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, ICC, IF
Biological background
Mitochondrial ATP synthase consists of a catalytic F1 domain and a membrane embedded F0 domain. ATP5MC1 belongs to the subunit c family of small hydrophobic proteins that form a ring structure in the F0 domain. This ring serves as the proton channel, allowing proton flow to power rotation of the central stalk, driving ATP synthesis. Research using ATP5MC1 antibody has revealed that this protein is essential for energy transduction, ensuring efficient conversion of electrochemical gradients into chemical energy. Without proper function, cells face energy deficits that compromise survival and function.
Mutations in ATP5MC1 and related subunits have been associated with mitochondrial disease, including encephalomyopathy, developmental delay, and neuromuscular weakness. Studies with ATP5MC1 antibody have demonstrated that defects disrupt assembly and function of ATP synthase, leading to decreased ATP production. Because high energy organs such as the brain, heart, and skeletal muscle depend heavily on oxidative phosphorylation, ATP5MC1 dysfunction has widespread clinical consequences. These findings underscore its importance in human health.
Beyond rare mitochondrial disorders, alterations in ATP synthase subunit expression have been linked to aging, neurodegeneration, and cancer. Research with ATP5MC1 antibody has shown that tumor cells can modulate expression of ATP synthase components to reprogram metabolism, balancing oxidative phosphorylation and glycolysis to support growth. In neurodegeneration, reduced ATP synthase function impairs neuronal survival and synaptic activity, contributing to cognitive decline. This positions ATP5MC1 as a valuable target for both basic and translational research.
ATP5MC1 antibody is widely used in western blotting, immunohistochemistry, and immunofluorescence. Western blotting confirms its abundance in mitochondria rich tissues, immunohistochemistry highlights distribution in energy demanding organs, and immunofluorescence demonstrates mitochondrial localization through colocalization with established mitochondrial markers. These applications make ATP5MC1 antibody indispensable in mitochondrial biology research.
By providing validated ATP5MC1 antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
