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| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human ATP7A was used as the immunogen for the ATP7A antibody. |
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Overview
ATP7A Antibody / Copper-transporting ATPase 1 is a anti-ATP7A Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS) with listed reactivity in Human.
Key elements and design rationale
- Target: ATP7A
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS
Biological background
Copper-transporting ATPase 1 is a multi-domain membrane protein containing ATP-binding and transmembrane copper-binding motifs that drive active ion transport using ATP hydrolysis. It localizes mainly to the trans-Golgi network under basal conditions but relocates to the plasma membrane during copper overload. The ATP7A antibody allows researchers to study this dynamic localization, revealing how cells adapt to fluctuating copper availability to maintain metal homeostasis. ATP7A activity ensures proper metallation of key enzymes such as lysyl oxidase, dopamine beta-hydroxylase, and superoxide dismutase 1.
Loss-of-function mutations in ATP7A cause Menkes disease, a lethal X-linked disorder characterized by systemic copper deficiency leading to neurodegeneration, connective tissue abnormalities, and growth retardation. The ATP7A antibody is crucial for diagnostic and mechanistic research into this condition, enabling detection of protein expression and distribution defects in patient-derived fibroblasts and tissues. Reduced expression or mislocalization of Copper-transporting ATPase 1 disrupts copper delivery to secretory enzymes, impairing multiple physiological processes.
In addition to Menkes disease, partial ATP7A dysfunction underlies occipital horn syndrome, a milder disorder affecting connective tissue and autonomic function. The ATP7A antibody supports comparative studies of these phenotypes and aids in identifying molecular defects in copper trafficking. Furthermore, ATP7A expression influences tumor progression and drug resistance, as copper transport impacts angiogenesis and the uptake of platinum-based chemotherapeutics.
The ATP7A antibody is validated for western blotting, immunohistochemistry, and immunofluorescence, showing clear perinuclear and membrane localization depending on copper conditions.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
