| Field | Specification |
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| Host | |
| Immunogen | E.coli-derived human ATF5 recombinant protein (Position: L11-R275) was used as the immunogen for the BBS1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
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| Target | |
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Overview
BBS1 Antibody / Bardet-Biedl syndrome 1 is a anti-BBS1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: BBS1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ELISA
Biological background
The BBSome complex, composed of at least eight core proteins including BBS1, BBS2, BBS4, and BBS7, mediates cargo recognition and vesicular transport along the ciliary membrane. Bardet-Biedl syndrome 1 protein acts as a structural hub that anchors the complex to the basal body and interacts with small GTPases such as ARL6. The BBS1 antibody allows visualization of this component in centrosomes and cilia, supporting studies into how BBSome assembly regulates signaling receptor localization and function.
Mutations in BBS1 are the most common cause of Bardet-Biedl syndrome, a pleiotropic ciliopathy characterized by retinal degeneration, obesity, renal anomalies, and polydactyly. These mutations impair BBSome stability and disrupt ciliary transport of G-protein-coupled receptors (GPCRs). The BBS1 antibody supports functional research into these mechanisms by enabling analysis of protein expression, localization, and complex formation in patient cells and model organisms. It is a critical tool for defining how ciliary trafficking defects lead to multisystemic disease manifestations.
Beyond ciliopathy research, Bardet-Biedl syndrome 1 protein has been implicated in metabolic regulation, immune signaling, and neural development. The BBS1 antibody supports studies exploring these expanded roles, including how BBSome components modulate leptin receptor and Hedgehog signaling pathways. Dysregulation of ciliary protein transport impacts sensory and hormonal signaling, linking BBS1 to diverse physiological processes.
The BBS1 antibody performs effectively in western blotting, immunofluorescence, and immunohistochemistry, revealing punctate centrosomal and ciliary staining patterns.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
