| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human BAP29/BCAP29 recombinant protein (Position: L64-L241) was used as the immunogen for the BCAP29 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
BCAP29 Antibody / B-cell receptor-associated protein 29 is a anti-BCAP29 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm (ER).
Key elements and design rationale
- Target: BCAP29
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC, IF, FACS, ELISA
Biological background
B-cell receptor-associated protein 29 contributes to proper folding and assembly of multimeric proteins in the ER. It interacts with several chaperone components, including Sec61 and calnexin, to ensure accurate post-translational processing before cargo delivery to the Golgi. The BCAP29 antibody supports localization studies demonstrating its distribution along ER membranes and cis-Golgi compartments. Through its cooperation with BCAP31, it links cargo transport with apoptotic signaling under stress conditions.
In B lymphocytes, B-cell receptor-associated protein 29 participates in antigen receptor processing and trafficking. The BCAP29 antibody enables investigation of these processes and provides insight into how ER quality control affects immune-receptor assembly. It is also expressed in non-immune cells, indicating a more general role in secretory-pathway regulation. Disruption of BCAP29 function has been associated with altered membrane trafficking and ER stress responses.
Emerging evidence suggests B-cell receptor-associated protein 29 may modulate apoptosis and calcium homeostasis. Its interaction with the pro-apoptotic regulator BCL2 family proteins suggests that BCAP29 may act as a mediator between ER stress and mitochondrial signaling. The BCAP29 antibody allows researchers to study these molecular connections in models of neurodegeneration, immune activation, and cancer.
The BCAP29 antibody performs effectively in western blotting, immunofluorescence, and immunohistochemistry, producing characteristic perinuclear staining consistent with ER localization.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
