| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human BAP31/BCAP31 recombinant protein (Position: M1-D242) was used as the immunogen for the BCAP31 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
BCAP31 Antibody / B-cell receptor-associated protein 31 is a anti-BCAP31 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: ER.
Key elements and design rationale
- Target: BCAP31
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, IF, ICC, IP, FACS, ELISA
Biological background
BCAP31 is encoded by the BCAP31 gene located on the X chromosome (Xp22.13). The protein is approximately 28 kilodaltons and contains three transmembrane domains with a cytoplasmic C-terminal tail that interacts with diverse partners, including BCL2 and procaspase-8. These interactions position BCAP31 at the intersection of the secretory and apoptotic pathways, integrating ER homeostasis with cell death regulation.
The BCAP31 antibody detects a 28 kilodalton band on western blot and shows reticular ER staining consistent with its localization. BCAP31 forms complexes with chaperones such as calnexin and Sec61, assisting in the folding and export of glycoproteins from the ER to the Golgi apparatus. It also mediates ER retention or degradation of misfolded proteins, linking it to the unfolded protein response (UPR).
In apoptosis, BCAP31 acts as a substrate for caspase cleavage, generating pro-apoptotic fragments that amplify death signaling through mitochondria. This dual role allows BCAP31 to serve as both a quality control checkpoint and a stress sensor. Mutations or deletions of BCAP31 cause X-linked intellectual disability syndromes characterized by developmental delay and hearing loss, emphasizing its importance in neuronal maintenance.
BCAP31 also contributes to immune cell signaling and antigen presentation by modulating trafficking of MHC class I molecules.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
