| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human BCCIP recombinant protein (Position: K167-K300) was used as the immunogen for the BCCIP antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
BCCIP Antibody / BRCA2 and CDKN1A-interacting protein is a anti-BCCIP Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear.
Key elements and design rationale
- Target: BCCIP
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC, IF, FACS, ELISA
Biological background
Structurally, BCCIP contains conserved coiled-coil domains and an acidic region that mediate protein-protein interactions with DNA repair and replication factors. It is expressed in proliferating cells and localizes primarily to the nucleus, particularly at sites of DNA damage. Through its association with p21, BCCIP also influences CDK activity and contributes to G1/S and G2/M checkpoint control.
The BCCIP antibody is widely used in DNA repair, oncology, and cell cycle research to study BRCA2-associated homologous recombination and genomic integrity. Western blot analysis identifies a 36 kilodalton band corresponding to BCCIP, while immunofluorescence shows nuclear punctate staining consistent with DNA damage foci. This antibody enables detection of repair complex dynamics and checkpoint activation in mammalian cells.
Dysregulation of BCCIP expression impairs DNA repair fidelity, leading to chromosomal instability, replication stress, and increased tumor susceptibility. Reduced BCCIP levels have been observed in glioblastoma and colorectal cancer, supporting its role as a tumor suppressor. The BCCIP antibody provides a reliable reagent for exploring DNA damage response and tumorigenic mechanisms.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
