| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human BCHE recombinant protein (Position: E29-L602) was used as the immunogen for the BCHE antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
BCHE Antibody / Butyrylcholinesterase is a anti-BCHE Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: BCHE
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ELISA
Biological background
Functionally, BCHE antibody identifies a 602-amino-acid glycoprotein secreted as a tetrameric enzyme that hydrolyzes choline esters such as butyrylcholine and succinylcholine. BCHE regulates cholinergic neurotransmission indirectly by hydrolyzing circulating esters and serves as a bioscavenger for organophosphorus compounds and nerve agents. It also participates in drug metabolism by inactivating esterified anesthetics and muscle relaxants.
The BCHE gene is located on chromosome 3q26.1-q26.2 and encodes a protein synthesized mainly in the liver before secretion into plasma. BCHE shares structural homology with acetylcholinesterase but differs in substrate specificity and inhibitor sensitivity. Its activity is regulated by genetic polymorphisms that influence catalytic efficiency and drug sensitivity. Reduced BCHE activity causes prolonged neuromuscular blockade following exposure to succinylcholine or mivacurium, a clinically relevant pharmacogenetic condition.
Beyond neurotransmission, BCHE plays roles in lipid metabolism, inflammation, and neurodegenerative diseases. Elevated BCHE activity is associated with obesity and metabolic syndrome, while decreased activity has been observed in liver dysfunction, Alzheimer's disease, and chronic inflammation. Its ability to hydrolyze ghrelin and other acylated hormones links it to appetite regulation and energy balance. In neurobiology, BCHE is thought to modulate cholinergic tone during aging and cognitive decline.
BCHE antibody is widely used in neurobiology, pharmacology, and metabolic disease research. It is suitable for western blotting, ELISA, and enzyme histochemistry to detect BCHE in plasma, liver, or neural tissue. This antibody supports studies of cholinesterase activity, drug metabolism, and neurodegenerative mechanisms. In toxicology, BCHE detection helps evaluate exposure to organophosphate inhibitors and nerve agents.
Structurally, BCHE is a glycoprotein composed of four identical subunits stabilized by disulfide bonds and carbohydrate moieties. Its catalytic triad (Ser-His-Glu) mediates ester hydrolysis through a charge-relay mechanism.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
