| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human BCL6 recombinant protein (Position: R28-K336) was used as the immunogen for the BCL6 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
BCL6 Antibody / B-cell lymphoma 6 is a anti-BCL6 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: BCL6
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
B-cell lymphoma 6 is required for the formation and maintenance of germinal centers during immune responses. It represses genes such as TP53 and PRDM1 to allow B cells to proliferate and undergo somatic hypermutation without triggering apoptosis. The BCL6 antibody allows researchers to visualize this dynamic regulation in lymphoid tissues and cell lines, offering insight into B-cell maturation and immune system adaptation. BCL6 expression is transiently induced during the germinal center stage and subsequently downregulated as cells differentiate into plasma or memory B cells.
Aberrant BCL6 expression is implicated in diffuse large B-cell lymphoma (DLBCL) and follicular lymphoma, where chromosomal translocations or mutations disrupt its normal regulation. These alterations lead to constitutive repression of target genes and uncontrolled proliferation. The BCL6 antibody serves as an important diagnostic and research tool for detecting abnormal expression in lymphoma biopsies and assessing therapeutic responses to BCL6 inhibitors. Its use in immunohistochemistry and western blotting allows pathologists to distinguish germinal center B-cell subtypes from activated or memory B cells.
Beyond hematologic malignancies, B-cell lymphoma 6 influences other tissues by modulating inflammation, metabolism, and neural differentiation. In macrophages and T cells, it acts as a transcriptional repressor that controls cytokine production and immune tolerance. The BCL6 antibody enables studies of these non-lymphoid functions, expanding understanding of how this transcription factor integrates immune and metabolic signaling networks. Recent research also links BCL6 to cancer stem cell maintenance and chemoresistance in solid tumors, underscoring its broad biological significance.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
