| Field | Specification |
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| Host | |
| Immunogen | A synthesized peptide derived from human BDKRB2 was used as the immunogen for the BDKRB2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
BDKRB2 Antibody / Bradykinin receptor B2 is a anti-BDKRB2 Rabbit antibody Recombinant Rabbit Monoclonal clone AFAG-2 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP) with listed reactivity in Human, Mouse.
Key elements and design rationale
- Target: BDKRB2
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone AFAG-2, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC, IP
Biological background
Functionally, BDKRB2 antibody identifies a 364-amino-acid transmembrane receptor that activates intracellular signaling cascades upon bradykinin binding. The B2 receptor couples primarily to Gq and Gi proteins, triggering phospholipase C activation, intracellular calcium release, and nitric oxide production. This cascade promotes vasodilation, increases vascular permeability, and contributes to pain sensitization. BDKRB2 also regulates smooth muscle contraction and endothelial function through modulation of cyclic GMP pathways.
The BDKRB2 gene is located on chromosome 14q32.1 and encodes a receptor with seven transmembrane helices typical of GPCRs. Unlike the inducible B1 receptor, BDKRB2 is constitutively expressed under physiological conditions and mediates most acute responses to bradykinin. The receptor undergoes rapid desensitization and internalization upon agonist stimulation, allowing precise control of signal duration and intensity. BDKRB2 signaling is critical for cardiovascular regulation, renal function, and nociception.
In the vascular system, BDKRB2 activation stimulates endothelial nitric oxide synthase (eNOS), leading to vasorelaxation and reduced blood pressure. In the kidney, it modulates sodium excretion and renin release, linking it to salt balance and blood pressure homeostasis. Dysregulation of BDKRB2 expression or signaling contributes to hypertension, hereditary angioedema, and chronic inflammatory conditions. Polymorphisms in BDKRB2 have been associated with altered cardiovascular responses and endurance performance in humans.
Beyond vascular biology, BDKRB2 participates in neuroinflammation, pain transmission, and tumor biology. Its activation sensitizes nociceptive neurons and facilitates cross-talk with cytokine and prostaglandin pathways. In cancer, BDKRB2 expression promotes angiogenesis and cell migration, suggesting a role in tumor progression. Conversely, BDKRB2-mediated nitric oxide release can induce apoptosis under oxidative conditions, reflecting its context-dependent activity.
BDKRB2 antibody is used extensively in research on inflammation, vascular signaling, and neurobiology. It is suitable for immunoblotting, immunofluorescence, and receptor binding assays to detect expression patterns and subcellular localization. This antibody aids in studying GPCR signaling mechanisms and the pharmacological modulation of bradykinin pathways.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
