Beta-Tubulin Monoclonal Antibody

SKU:BHA12111408
Overview
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TUBB3-specific monoclonal antibody raised in Mouse. Validated for Western Blot in Zebrafish, Fulvidraco, Blowfish samples. Affinity-purified rabbit IgG.
Specificity Total Protein
Immunogen Synthetic Peptide
Molecular Weight ~50.4 kDa
UniProt Q13509
Gene ID Human: 10381; Mouse: 22152; Rat: 246118
Applications 1 validated
Options selector
Catalog no. Size
BT-MCA1344-20UL 20 uL
BT-MCA1344-50UL 50 uL
BT-MCA1344-100UL 100 uL
Available Options
Size
Field Specification
Mfr No BT-MCA1344
Alternative Names TUBB3; TUBB4; Tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-III
Clonality
  • Monoclonal
Concentration 1 mg/ml
Formulation PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Gene ID Human: 10381; Mouse: 22152; Rat: 246118
Host Mouse
Immunogen Synthetic Peptide of Beta-Tubulin
Isotype
  • IgG
Molecular Weight ~50.4 kDa
Product Type
  • Primary Antibody
Reactivity
  • Zebrafish
  • Fulvidraco
  • Blowfish
Storage -20°C for 1 year
Target TUBB3

Product Overview

Primary Antibody

This antibody targets TUBB3 and detects endogenous levels of TUBB3 protein.

Validated Applications

Application Dilution Guidance
Western Blot (WB) Refer to datasheet for recommended working dilution
✔ Validated by Bioassay Technology Laboratory

Antibody Specifications

Target TUBB3
Host Mouse
Clonality Monoclonal
Isotype IgG
Reactivity Zebrafish, Fulvidraco, Blowfish
Purification Affinity Purification
Molecular Weight ~50.4 kDa
Concentration 1 mg/ml
Immunogen Synthetic Peptide of Beta-Tubulin
Formulation PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Storage -20°C for 1 year
Gene ID Human: 10381; Mouse: 22152; Rat: 246118
UniProt Q13509 (Human: Q13509; Mouse: Q9ERD7; Rat: Q4QRB4)

Scientific Background

This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6.

Alternative Names

TUBB3; TUBB4; Tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-III

Safety & Handling

⚠ Research Use Only (RUO). Not approved for diagnostic or therapeutic use. Handle with standard laboratory safety precautions. Contains sodium azide — avoid contact with skin/eyes; dispose per local regulations.
TargetTUBB3
HostMouse
ClonalityMonoclonal
IsotypeIgG
ReactivityZebrafish, Fulvidraco, Blowfish
PurificationAffinity Purification
Molecular Weight~50.4 kDa
Concentration1 mg/ml
ImmunogenSynthetic Peptide of Beta-Tubulin
FormulationPBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Storage-20°C for 1 year
Gene IDHuman: 10381; Mouse: 22152; Rat: 246118
UniProtQ13509 (Human: Q13509; Mouse: Q9ERD7; Rat: Q4QRB4)

Optimized working dilutions depend on sample type and detection system. Titration is recommended.

ApplicationDilution Note
Western Blot (WB)Refer to datasheet for recommended working dilution

This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6.

Alternative Names

TUBB3; TUBB4; Tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-III

What species does this antibody react with?
This antibody has been validated for reactivity against Zebrafish, Fulvidraco, Blowfish. Actual cross-reactivity is determined by immunogen sequence homology across species. Contact technical support for unlisted species.
What applications has this antibody been validated for?
Validated applications include: Western Blot (WB). Each application was tested with positive and negative controls. Contact our team regarding applications not listed.
How was this antibody produced and purified?
This monoclonal IgG antibody was raised in rabbit against a synthesized target-specific peptide. It was affinity-purified from antiserum using an epitope-specific immunogen, yielding a highly specific antibody suitable for multiple research applications.
What is the expected molecular weight of TUBB3?
The predicted molecular weight of TUBB3 is ~50.4 kDa. Band size may vary slightly depending on post-translational modifications and gel percentage.
What storage conditions does this antibody require?
Store at −20°C for long-term storage. Avoid repeated freeze-thaw cycles. Working aliquots can be stored at 4°C for short-term use. The formulation includes 50% glycerol as a cryoprotectant.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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