BGAT Rabbit Polyclonal Antibody

SKU:BHA12107555
Overview
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ABO-specific polyclonal antibody raised in Rabbit. Validated for Western Blot in Human, Mouse samples. Affinity-purified rabbit IgG.
Specificity Total Protein
Immunogen Synthetic Peptide
UniProt P16442
Gene ID Human: 28; Mouse: 80908
Applications 1 validated
Options selector
Catalog no. Size
BT-AP06345-20UL 20 uL
BT-AP06345-50UL 50 uL
BT-AP06345-100UL 100 uL
Available Options
Size
Field Specification
Mfr No BT-AP06345
Alternative Names BGAT; Histo-blood group ABO system transferase; Fucosylglycoprotein 3-alpha-galactosyltransferase; Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase; Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase; EC 2.4.1.40; Glycoprotein-fucosylgalactoside alpha-galactosyltransferase; EC 2.4.1.37; Histo-blood group A transferase; A transferase; Histo-blood group B transferase; B transferase; NAGAT; Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase soluble form;
Clonality
  • Polyclonal
Concentration 1 mg/ml
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.47% sodium azide.
Gene ID Human: 28; Mouse: 80908
Host Rabbit
Immunogen Synthesized peptide derived from human BGAT
Isotype
  • IgG
Product Type
  • Primary Antibody
Reactivity
  • Human
  • Mouse
Storage -20°C for 1 year
Target ABO

Product Overview

Primary Antibody

This antibody targets ABO and detects endogenous levels of ABO protein.

Validated Applications

Application Dilution Guidance
Western Blot (WB) Refer to datasheet for recommended working dilution
✔ Validated by Bioassay Technology Laboratory

Antibody Specifications

Target ABO
Host Rabbit
Clonality Polyclonal
Isotype IgG
Reactivity Human, Mouse
Purification Affinity Purification
Concentration 1 mg/ml
Immunogen Synthesized peptide derived from human BGAT
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.47% sodium azide.
Storage -20°C for 1 year
Gene ID Human: 28; Mouse: 80908
UniProt P16442 (Human: P16442; Mouse: P38649)

Scientific Background

This gene encodes proteins related to the first discovered blood group system, ABO. Which allele is present in an individual determines the blood group. The 'O' blood group is caused by a deletion of guanine-258 near the N-terminus of the protein which results in a frameshift and translation of an almost entirely different protein. Individuals with the A, B, and AB alleles express glycosyltransferase activities that convert the H antigen into the A or B antigen. Other minor alleles have been found for this gene.

Alternative Names

BGAT; Histo-blood group ABO system transferase; Fucosylglycoprotein 3-alpha-galactosyltransferase; Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase; Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase; EC 2.4.1.40; Glycoprotein-fucosylgalactoside alpha-galactosyltransferase; EC 2.4.1.37; Histo-blood group A transferase; A transferase; Histo-blood group B transferase; B transferase; NAGAT; Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase soluble form;

Safety & Handling

⚠ Research Use Only (RUO). Not approved for diagnostic or therapeutic use. Handle with standard laboratory safety precautions. Contains sodium azide — avoid contact with skin/eyes; dispose per local regulations.
TargetABO
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ReactivityHuman, Mouse
PurificationAffinity Purification
Concentration1 mg/ml
ImmunogenSynthesized peptide derived from human BGAT
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.47% sodium azide.
Storage-20°C for 1 year
Gene IDHuman: 28; Mouse: 80908
UniProtP16442 (Human: P16442; Mouse: P38649)

Optimized working dilutions depend on sample type and detection system. Titration is recommended.

ApplicationDilution Note
Western Blot (WB)Refer to datasheet for recommended working dilution

This gene encodes proteins related to the first discovered blood group system, ABO. Which allele is present in an individual determines the blood group. The 'O' blood group is caused by a deletion of guanine-258 near the N-terminus of the protein which results in a frameshift and translation of an almost entirely different protein. Individuals with the A, B, and AB alleles express glycosyltransferase activities that convert the H antigen into the A or B antigen. Other minor alleles have been found for this gene.

Alternative Names

BGAT; Histo-blood group ABO system transferase; Fucosylglycoprotein 3-alpha-galactosyltransferase; Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase; Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase; EC 2.4.1.40; Glycoprotein-fucosylgalactoside alpha-galactosyltransferase; EC 2.4.1.37; Histo-blood group A transferase; A transferase; Histo-blood group B transferase; B transferase; NAGAT; Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase soluble form;

What species does this antibody react with?
This antibody has been validated for reactivity against Human, Mouse. Actual cross-reactivity is determined by immunogen sequence homology across species. Contact technical support for unlisted species.
What applications has this antibody been validated for?
Validated applications include: Western Blot (WB). Each application was tested with positive and negative controls. Contact our team regarding applications not listed.
How was this antibody produced and purified?
This polyclonal IgG antibody was raised in rabbit against a synthesized target-specific peptide. It was affinity-purified from antiserum using an epitope-specific immunogen, yielding a highly specific antibody suitable for multiple research applications.
What is the expected molecular weight of ABO?
The expected molecular weight of ABO can be found in the product datasheet. Band size may vary slightly depending on post-translational modifications and gel percentage.
What storage conditions does this antibody require?
Store at −20°C for long-term storage. Avoid repeated freeze-thaw cycles. Working aliquots can be stored at 4°C for short-term use. The formulation includes 50% glycerol as a cryoprotectant.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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