| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived mouse Bim/Bcl2l11 recombinant protein (Position: Q18-R186) was used as the immunogen for the Bim antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Bim Antibody / Bcl-2-like protein 11 is a anti-BCL-2-LIKE Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Mouse.
Key elements and design rationale
- Target: BCL-2-LIKE
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
Functionally, Bim antibody identifies a 198-amino-acid cytoplasmic protein that binds anti-apoptotic Bcl-2 proteins (such as BCL2, BCL-XL, and MCL1), releasing pro-apoptotic effectors BAX and BAK to trigger mitochondrial cytochrome c release and caspase activation. Bim exists in several isoforms (BimEL, BimL, and BimS) generated by alternative splicing, which modulate apoptosis sensitivity across cell types.
The BCL2L11 gene is located on chromosome 2q13 and is expressed in lymphoid, neuronal, and epithelial tissues. Its expression is tightly regulated by survival pathways including PI3K/AKT and MAPK signaling, which control phosphorylation and proteasomal degradation of Bim.
Pathologically, dysregulation of Bim contributes to cancer, autoimmune diseases, and neurodegeneration. Reduced Bim expression confers resistance to apoptosis in tumor cells, while overexpression causes excessive neuronal death. Research using Bim antibody supports studies in cell death signaling, oncology, and stress-induced apoptosis.
Bim antibody is validated for western blotting, immunohistochemistry, and apoptosis assays to detect Bcl-2 family proteins.
Structurally, Bcl-2-like protein 11 contains a BH3 domain required for binding to anti-apoptotic Bcl-2 members and activating the intrinsic apoptotic pathway. This antibody supports exploration of Bim's role in stress-induced mitochondrial apoptosis and cellular homeostasis.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
