| Field | Specification |
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| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human BIN1 was used as the immunogen for the BIN1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
BIN1 Antibody / Bridging integrator 1 is a anti-BIN1 Rabbit antibody Recombinant Rabbit Monoclonal clone 30B93 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow cytometry (FACS) with listed reactivity in Human.
Key elements and design rationale
- Target: BIN1
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 30B93, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC, ICC, IF, IP, FACS
Biological background
BIN1 antibody is highly relevant for research into neuromuscular disorders, cancer, and Alzheimer disease. Mutations in BIN1 are associated with centronuclear myopathy, a congenital muscle disorder characterized by abnormal nuclei positioning and impaired muscle function. Genome wide association studies have also linked BIN1 to increased risk of Alzheimer disease, suggesting that altered BIN1 expression or function contributes to neurodegeneration. In oncology, BIN1 has been identified as a tumor suppressor, where reduced expression correlates with enhanced proliferation and invasion.
The antibody is suitable for western blotting, immunohistochemistry, immunofluorescence, and flow cytometry. BIN1 antibody detects protein bands corresponding to different isoforms, which arise from extensive alternative splicing. In immunohistochemistry, the antibody highlights tissue specific localization, including strong signals in muscle fibers and neuronal tissues. Immunofluorescence enables detailed visualization of BIN1 distribution along membranes and T tubules, reflecting its role in membrane remodeling.
BIN1 is also a key regulator of signaling pathways. It interacts with c Myc and modulates transcriptional activity, contributing to its tumor suppressor function. By detecting BIN1 expression with specific antibodies, researchers can explore how changes in BIN1 contribute to malignant transformation. In neuroscience, altered BIN1 expression is thought to affect tau pathology and amyloid processing, making it an important candidate in Alzheimer disease research.
BIN1 antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
