| Field | Specification |
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| Host | |
| Immunogen | A synthesized peptide derived from human BIN1 was used as the immunogen for the BIN1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
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| Target | |
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Overview
BIN1 Antibody / Bridging integrator 1 is a anti-BIN1 Rabbit antibody Recombinant Rabbit Monoclonal clone 30B92 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS) with listed reactivity in Human, Mouse.
Key elements and design rationale
- Target: BIN1
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 30B92, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC, ICC, IF, FACS
Biological background
BIN1 antibody is widely used in neuroscience, muscle biology, and oncology. In neurons, BIN1 regulates synaptic vesicle endocytosis and interacts with tau protein, linking it to Alzheimer disease. In muscle cells, BIN1 supports T tubule formation and excitation contraction coupling, where its loss results in centronuclear myopathy. Altered BIN1 expression has also been implicated in cancer, where it acts as a tumor suppressor by modulating MYC signaling. By detecting BIN1, researchers can study how membrane remodeling proteins influence physiology and disease.
In western blot assays, BIN1 antibody detects isoforms of varying molecular weight across tissues. Immunohistochemistry maps BIN1 expression in brain, skeletal muscle, and heart, while immunofluorescence highlights localization at endocytic sites and T tubules. ELISA applications support quantification in experimental systems.
In Alzheimer disease, BIN1 variants are among the strongest genetic risk factors identified in genome wide association studies. BIN1 interacts with tau and endocytic machinery, contributing to pathological tau propagation and neurodegeneration. By applying BIN1 antibody, scientists can explore mechanisms linking endocytosis to dementia. In muscle biology, BIN1 mutations lead to defective T tubule biogenesis, disrupting calcium handling and muscle contraction. BIN1 antibody is therefore essential for both basic and translational research into neuromuscular disease.
BIN1 also functions in immune regulation and tumor suppression. Reduced BIN1 expression correlates with cancer progression and poor prognosis, highlighting its role as a negative regulator of oncogenic signaling. Its diverse functions across tissues make BIN1 antibody valuable for multiple research areas.
BIN1 antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
