Biotinylated Histone H3 Peptide Substrate

What it means: — this protein was produced in , determining its post-translational modification profile and application suitability.

Why it matters:

• Insect cell (Sf9) systems support post-translational processing required for kinase activity and complex formation; HEK293 supports human-like glycosylation; E. coli yields high-quantity non-glycosylated protein.
• For assays measuring native enzymatic activity (kinase, phosphodiesterase, deacetylase), the expression system directly affects the quality and accuracy of results.

How to interpret here: Verify is compatible with your assay requirements, particularly for enzyme kinetics and cell-based applications.

What it means: >95% — protein purity was determined by SDS-PAGE and/or HPLC to confirm the proportion of correctly-sized target protein in the preparation.

Why it matters:

• High purity is critical for enzyme kinetics, inhibitor IC50 determinations, and SPR/BLI where contaminants would introduce signal artefacts or change the apparent kinetic parameters.
• Consistent lot-to-lot purity underpins reproducibility in hit-to-lead screening campaigns.

How to interpret here: >95% as determined by SDS-PAGE. Lot-specific QC data is provided on the Certificate of Analysis with each shipment.

What it means: Not applicable (BSL-1) — this protein has been classified under BSL-1 safety requirements, the lowest biosafety classification.

Why it matters:

• BSL-1 proteins pose no known risk to healthy adult laboratory workers and do not require specialised containment or personal protective equipment beyond standard laboratory practice.
• Confirms suitability for use in standard biochemistry or molecular biology laboratories without institutional BSL-2 or higher facility requirements.

How to interpret here: Standard laboratory safety practices apply. Review the product SDS/Datasheet for handling precautions specific to this compound.