| Field | Specification |
|---|---|
| Mfr No | |
| Assay Time | |
| Detection Range | |
| Gene ID | |
| Product Type | |
| Reactivity | |
| Sample Type(s) | Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates, Other Biological Fluids |
| Sensitivity | |
| Storage | |
| UniProt # |
Assay Principle
AP (Aprotinin) is a biomarker protein expressed in bovine tissues and biological fluids. Dysregulation of Aprotinin has been implicated in cellular signaling and fundamental cell biology, making it a relevant biomarker for investigational studies.
Quantitative measurement of Aprotinin enables researchers to characterize its abundance in Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant and correlate levels with experimental conditions. Studies investigating Aprotinin have contributed to the understanding of cell signaling pathways, disease pathogenesis, and therapeutic target validation.
This competitive ELISA kit provides a validated, reproducible format for Aprotinin quantification within the inhibition range of 1.56-100ng/mL. The assay supports experimental workflows in Cell Biology research.
Kit Components
Each kit contains sufficient reagents for one 96-well plate (approximately 40 samples in duplicate with standard curve):
Pre-coated microplate (96 wells), HRP-conjugated antigen, standard protein, sample diluent, wash buffer, TMB substrate solution, stop solution, and product manual.
Validated LotPerformance Data
| Assay Type | Competitive ELISA |
|---|---|
| Detection Range | 1.56-100ng/mL |
| Sensitivity (LLOD) | 0.43ng/mL |
| Validated Sample Types | Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant |
| Total Assay Time | 2.5 hours |
| Detection Method | Colorimetric (450 nm / 540–570 nm reference) |
| Plate Format | 96-well microplate |
| Reactivity | Bovine |
| UniProt Accession | P00974 |
| NCBI Gene ID | 404172 |
| Storage | The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit |
Assay Procedure Summary
Add samples and HRP-conjugated antigen simultaneously to pre-coated wells and incubate. Wash, add TMB substrate, and read at 450 nm. Signal is inversely proportional to AP concentration. Construct a standard inhibition curve from the provided reference antigen to calculate sample concentrations. Total protocol time: approximately 2.5 hours.
Pre-warm all kit components to room temperature (18–25 °C) for 30 minutes before starting. Run all samples in duplicate. Centrifuge samples at ≥1,000 × g before use to remove particulates.
Safety & Intended Use
Intended UseResearch Use Only (RUO)VendorSt John's Laboratory LtdCatalog No.STJE0013569SpeciesBovineUniProtP00974
This kit is validated for serum and plasma. Centrifuge samples at ≥1,000 × g for 15 min to remove particulates. Avoid hemolyzed, lipemic, or heat-inactivated samples. As a competitive format, higher analyte concentration produces lower signal — dilute proportionally. Pre-dilute samples within the linear range before committing full plates.
Minimum detectable concentration: 0.43ng/mL. Quantitative standard curve range: 1.56-100ng/mL. Samples above the upper limit must be diluted; near-lower-limit samples require duplicate measurement.
Total protocol time is approximately 2.5 hours of hands-on and incubation time. Pre-warm all reagents and prepare dilution series in advance. Set up a timer for each incubation step to maintain consistent timing across plates.
A standard microplate spectrophotometer set to 450 nm (reference: 540–570 nm) is required. An automated plate washer reduces wash-step variability. Calibrate and warm up your reader before measuring. Blank using the zero-standard or buffer-only well before constructing the standard curve.
Recommended storage: The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to co. Reconstituted standard is stable at 4 °C for ≤1 week; prepare fresh for critical experiments.
Looking for a custom format, bulk quantity, or specialized formulation? We offer flexible sourcing options. Contact us to discuss your specific requirements.
