| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human BRAT1 was used as the immunogen for the BRAT1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
BRAT1 Antibody / BRCA1 associated ATM activator 1 is a anti-BRAT1 Rabbit antibody Recombinant Rabbit Monoclonal clone 29B86 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow cytometry (FACS) with listed reactivity in Human.
Key elements and design rationale
- Target: BRAT1
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 29B86, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, ICC, IF, IP, FACS
Biological background
BRAT1 antibody is widely used in DNA damage research, cancer biology, and neurology. Mutations in BRAT1 cause autosomal recessive neurodevelopmental disorders characterized by seizures, microcephaly, and developmental delay. By detecting BRAT1, researchers can explore how DNA damage response proteins maintain genomic stability and neuronal health.
Western blot assays identify BRAT1 protein bands in nuclear and mitochondrial extracts. Immunohistochemistry highlights expression in proliferative and neural tissues, while immunofluorescence reveals nuclear and cytoplasmic localization. These methods provide robust tools for studying BRAT1 distribution and function.
BRAT1 interacts with BRCA1, ATM, and DNA-PKcs, integrating multiple DNA repair pathways. Dysregulation of BRAT1 activity contributes to defective checkpoint signaling, apoptosis, and impaired mitochondrial function. In cancer research, BRAT1 has been linked to tumor suppression, but context-dependent roles suggest complex regulation. By applying BRAT1 antibody, scientists can study its roles in DNA repair, tumorigenesis, and neurological disease.
BRAT1 antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
