C57BL/6 Mouse Primary Pulmonary Vein Fibroblasts

SKU:BHC16400540
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    Overview
    Click light‑blue chips for details
    C57BL/6 Mouse Primary Pulmonary Vein Fibroblasts are primary fibroblasts derived from mouse c57bl/6 pulmonary vein and supplied in selectable cryopreserved and flask formats. Commonly used in cell culture, imaging, and immunofluorescence studies.
    Species Mouse
    Cell Type Fibroblasts
    Organ System Cardiovascular
    Tissue C57BL/6
    Morphology Fibroblast-like
    Available Options

    Select the variant that best fits your experiment. Availability and lead time may vary by option.

    • Options: Format (3) - Frozen Vial (0.5 x 10^6 cells), T25 Flask, T75 Flask
    • Lead time: options listed in "Availability Content"; otherwise, there will be a column of “lead time”, other statuses may take longer.
    • Storage: Cryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.
    • Shipping: Cryopreserved cells are shipped with dry ice overnight.
    • Upon receipt: store at the recommended temperature as soon as possible.
    • Sales terms and conditions: Please review prior to ordering.
    Options selector
    Catalog no. Format
    C57-6078-FV-0.5M Frozen Vial (0.5 x 10^6 cells)
    C57-6078-T25 T25 Flask
    C57-6078-T75 T75 Flask
    Field Specification
    Alternative Names C57PPVFB
    Product Type
    • Cells
    • Primary Cells
    Shipping Cryopreserved cells are shipped with dry ice overnight.
    Species Mouse
    Storage Cryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.

    Overview

    C57BL/6 Mouse Primary Pulmonary Vein Fibroblasts are primary fibroblasts derived from mouse c57bl/6 pulmonary vein. Product metadata indicate adherent growth with fibroblast-like morphology and a reported BSL-2 handling context. Depending on the selected variant, the product may be supplied in selectable cryopreserved and flask formats (Frozen Vial (0.5 x 10^6 cells), T25 Flask, and T75 Flask) for in vitro studies that benefit from tissue-relevant cellular context.

    Key elements and design rationale

    • Source and identity reflect the stated fibroblasts model and tissue origin, supporting experiments where cardiovascular context matters.
    • Reported classifications and phenotype descriptors include primary, adherent growth, and fibroblast-like morphology.
    • Selectable variants can include Frozen Vial (0.5 x 10^6 cells), T25 Flask, and T75 Flask; choose the listed format according to culture scale, handling preference, and downstream assay design.
    • Handling considerations should follow institutional practice appropriate for the reported BSL-2 classification and the datasheet associated with the selected format.

    Review the specification table and variant selector together when choosing the appropriate format for assay scale, tissue context, and downstream readouts.

    Biological background

    Fibroblasts help maintain extracellular matrix and stromal architecture while responding to inflammatory, mechanical, and profibrotic signals. Tissue-specific fibroblast programs are widely studied in wound repair, fibrosis, matrix remodeling, and paracrine signaling. In this case, the stated source material and cardiovascular context can influence morphology, baseline signaling, and assay responsiveness.

    Research relevance and current trends

    • Vascular biology remains an active area for experiments that compare tissue-specific phenotype, pathway response, or functional readouts in culture-based systems.
    • Fibrosis & ECM remains an active area for experiments that compare tissue-specific phenotype, pathway response, or functional readouts in culture-based systems.
    • Multiparametric readouts such as cell culture, imaging, and immunofluorescence are commonly combined to connect morphology, phenotype, and pathway-level response.

    Common research applications

    • Cell culture and condition-optimization studies to assess morphology, growth behavior, or baseline phenotype over time.
    • Imaging-based workflows to track morphology, localization, marker expression, cell-cell interactions, or reporter-linked signal.

    Changes in morphology, marker expression, proliferation, migration, barrier properties, reporter activity, or secreted factors are typically interpreted alongside matched controls and the selected culture conditions.

    Notes for experimental interpretation

    • Potential confounders include donor-to-donor variability, passage-dependent phenotypic drift, substrate effects, serum or media composition, and differences between cryopreserved and expansion-stage material.
    • Use matched controls and confirm identity with morphology- and marker-based readouts suited to the stated cell type, tissue source, and downstream assay.

    SKU:BHC16400540

    Customization & Add-ons: Can't find the cell line you need—or require a custom cell-based solution for your project? We can help you source the best match or support custom cell line services for diverse research needs, including cell line sourcing and selection (species, tissue, and disease model matching), stable cell line engineering (overexpression, knockdown, or knockout via CRISPR/Cas9, shRNA, or sgRNA), reporter gene integration (GFP, RFP, luciferase, and other fluorescent or bioluminescent constructs), genome editing and knockin (point mutations, tagged endogenous proteins, conditional alleles), inducible expression systems (Tet-On/Off and other regulatable constructs), drug resistance marker selection (puromycin, G418, hygromycin, and others), custom growth and media optimisation for specific assay requirements, scale-up production for high-throughput screening campaigns, and authentication and QC services (STR profiling, mycoplasma testing, viability assessment). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

    The Role of MicroRNA-21 in Venous Neointimal Hyperplasia: Implications for Targeting miR-21 for VNH Treatment

    Authors: Sreenivasulu Kilari,1 Chuanqi Cai,1,2 Chenglei Zhao,1,3 Amit Sharma,1 Ekaterina Chernogubova,4 Michael Simeon,1 Chin-Cheng Wu,5 Hsiang-Lin Song,6 Lars Maegdefessel,4 and Sanjay Misra1,7 Journal: Mol Ther Issue: 2019 Sep 4; 27(9): 1

    What growth behavior or morphology should I expect?
    These cells are reported as adherent with fibroblast-like morphology. Use that information to plan attachment conditions, imaging expectations, and routine culture workflow, while still confirming actual behavior after recovery in your own system.
    What should I confirm before ordering or starting an experiment?
    Before ordering, confirm the match between Mouse, C57BL/6 Pulmonary Vein, Primary, and Vascular biology and your assay design. It is also worth checking the selected format (Frozen Vial (0.5 x 10^6 cells), T25 Flask, and T75 Flask) aligns with your seeding plan, expansion needs, markers, and downstream readouts.

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