| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human Calpain 6/CAPN6 recombinant protein (Position: M1-L641) was used as the immunogen for the CAPN6 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
CAPN6 Antibody / Calpain 6 is a anti-CAPN6 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm, perinuclear.
Key elements and design rationale
- Target: CAPN6
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC/IF, ELISA
Biological background
One of the primary functions of Calpain 6 is to stabilize microtubules by directly binding to tubulin. Through this interaction, CAPN6 regulates lamellipodia formation and enhances the ability of cells to migrate. This cytoskeletal remodeling is essential for embryonic tissue development, wound healing, and angiogenesis. In endothelial cells, CAPN6 supports vessel formation and vascular integrity, while in osteoclasts it influences bone resorption. Researchers investigating structural dynamics of the cytoskeleton use CAPN6 antibody to trace its expression and monitor its interactions with microtubule networks.
The absence of proteolytic activity makes Calpain 6 unique among calpains, highlighting the functional diversity of this protein family. Instead of cleaving substrates, CAPN6 serves as a structural regulator, linking calcium signaling with cytoskeletal architecture. This distinction is particularly important in oncology, where abnormal expression of CAPN6 has been reported in hepatocellular carcinoma, uterine leiomyosarcoma, and endometrial cancer. Overexpression may enhance invasiveness by promoting cytoskeletal plasticity, while downregulation may disrupt normal development and tissue repair. The dual roles of CAPN6 underscore its importance as both a developmental regulator and a potential biomarker of malignancy.
CAPN6 antibody is versatile in laboratory applications. Western blotting can be used to detect protein levels under conditions that affect cytoskeletal dynamics, such as drug treatments or genetic manipulation. Immunofluorescence allows visualization of CAPN6 co-localization with tubulin, offering insight into how it modulates microtubule stability. Immunohistochemistry with CAPN6 antibody reveals distribution patterns in tumors and placental tissues, enabling researchers to evaluate associations between CAPN6 expression and disease progression. In developmental biology, CAPN6 antibody helps clarify the role of non-proteolytic calpains in tissue morphogenesis and angiogenesis.
The CAPN6 antibody provided by
As the only calpain family member lacking enzymatic activity, Calpain 6 occupies a unique position in protein biology. Its ability to regulate cell migration and cytoskeletal remodeling without protease function has expanded understanding of how calpains contribute to physiology and disease. By employing CAPN6 antibody, researchers can investigate how this protein influences endothelial biology, osteoclast activity, tumor invasion, and placental function. These insights not only clarify the role of CAPN6 in normal development but also suggest therapeutic relevance in oncology and regenerative medicine. For laboratories studying the intersection of cytoskeletal dynamics and cellular signaling, CAPN6 antibody remains an essential resource, supported by the consistent quality of
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
