| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived mouse CDKN2AIP/CARF recombinant protein (Position: R183-T689) was used as the immunogen for the Carf antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Carf Antibody / Collaborator of ARF / Cdkn2aip is a anti-ARF Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Mouse, Rat.
Key elements and design rationale
- Target: ARF
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
Structurally, CARF contains a coiled-coil domain and nuclear localization signals that facilitate interaction with chromatin-associated proteins and transcriptional regulators. It belongs to the CDKN2A-interacting protein family and acts as a bridge between the ARF tumor suppressor pathway and p53 signaling. CARF directly binds p14ARF and modulates p53 stability through MDM2 regulation, coordinating cell cycle arrest in response to genotoxic stress. Co-localization studies show CARF within the nucleus and nucleolus, where it associates with replication machinery and chromatin-modifying complexes.
Functionally, CARF serves as a cell cycle regulator that maintains p53 activity and prevents uncontrolled proliferation. Under DNA damage conditions, CARF enhances p53-dependent transcription of target genes such as CDKN1A (p21), promoting cell cycle arrest and senescence. It also regulates replication stress responses by interacting with checkpoint proteins such as ATR and CHK1. CARF expression levels determine whether a cell undergoes reversible arrest or permanent senescence, integrating multiple stress and growth signals.
Dysregulation of CARF expression has been implicated in cancer and aging. Overexpression can suppress proliferation and induce senescence, while loss of CARF may impair p53 function and enhance oncogenic transformation. In certain tumors, CARF mislocalization correlates with altered cell cycle control. Pathway associations include p53 signaling, DNA damage response, and cell cycle checkpoint regulation. Developmentally, CARF contributes to tissue homeostasis by regulating stem and progenitor cell proliferation.
The Carf antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
