| Field | Specification |
|---|---|
| Product Type | |
| Shipping | |
| Source | Recombinant (E. coli) |
| Storage |
Cas9 Nuclease is derived from the wild-type Streptococcus pyogenes and is an RNA-guided endonuclease that specifically cleaves double-stranded DNA (it can also cleave single-stranded DNA or RNA in the presence of a DNA PAM). The Cas9 cleavage site is located within the target sequence, three base pairs away from the PAM (NGG) region. Cas9 Nuclease has undergone codon optimization and design with a nuclear localization signal (NLS), and is expressed through recombinant expression in Escherichia coli. It exhibits high editing efficiency and can be used for gene modification in cells (such as hematopoietic stem cells, T cells, etc.), as well as for molecular diagnostics and pathogen detection.
Features
Optimized nuclear localization signal: The optimized nuclear localization signal (NLS) enhances editing efficiency.
High editing efficiency: Consistently high editing efficiency both in vitro and in vivo.
High purity: The purity is greater than or equal to 95%.
High concentration: It is applicable under standard editing conditions and is also suitable for optimizing editing conditions in more challenging scenarios, such as in primary or embryonic cells, through microinjection or when conducting high-throughput screening of multiple gRNA sequences.
Applications
CRISPR/Cas gene editing
Diagnostic and detection based on the CRISPR/Cas system
Specifications
|
Source |
The Cas9 gene from Streptococcus pyogenes is expressed through recombinant expression in Escherichia coli |
|
Molecular weight |
163 KDa |
|
Concentration |
10 mg/mL |
|
Purity |
≥95% |
|
Tag |
His |
|
Endotoxin |
≤10 EU/mg |
Glycerol Content |
Contains Glycerol |
Components
|
Components No. |
Name |
14701ES60 |
14701ES76 |
14701ES03 |
|
14701-A |
Cas9 Nuclease（10 mg/mL） |
10 μL |
50 μL |
100 μL |
|
14701-B |
10×Cas9 Nuclease Reaction Buffer |
500 μL |
1 mL |
1 mL |
Shipping and Storage
This product should be stored at -25 ~ -15oC for 1 years.
Figures
Figure 1. Results of Cas9 nuclease in vitro cleavage activity and gene knockout efficiency
Store this enzyme at -20°C and avoid repeated freeze-thaw cycles to preserve catalytic activity. The product is shipped Dry Ice and remains stable for up to one year from the date of manufacture when stored under recommended conditions. Aliquoting the stock solution into single-use volumes is recommended for enzymes used infrequently to minimize thermal cycling of the bulk stock.
CRISPR/Cas gene editing Diagnostic and detection based on the CRISPR/Cas system. Always verify compatibility with your specific template, buffer, and downstream workflow.
Nuclease activity is expressed in µg/µL; cleavage efficiency is validated as the percentage of target DNA cut within 60 min at 37°C using a defined guide RNA and target plasmid substrate.
This enzyme is produced as Recombinant (E. coli) and supplied as a Research Use Only (RUO) reagent. Each lot is subjected to activity assay, purity assessment by SDS-PAGE, and functional validation prior to release. A Certificate of Analysis (CoA) and Safety Data Sheet (SDS) are available on request.
On-target efficiency is primarily determined by guide RNA (gRNA) design, target accessibility within chromatin, and PAM sequence context. Off-target cleavage is influenced by sequence complementarity mismatches (particularly in the seed region proximal to the PAM), enzyme concentration, and delivery method. High-fidelity Cas9 variants with engineered positional charge substitutions significantly reduce off-target activity (>99% reduction) while maintaining on-target cleavage efficiency comparable to wild-type enzyme.
Yeasen Biotechnology supports custom enzyme solutions across multiple service lines — from GMP-grade bulk supply to directed enzyme engineering. Contact BioHippo to discuss requirements and initiate a project inquiry.
▶ GMP-Grade & Bulk Supply
Select Yeasen enzymes are available in GMP grade, manufactured in an ISO 13485-certified UCF.ME™ ultra-clean molecular enzyme facility with FDA Drug Master File (DMF) support.
- GMP-grade release testing and CoA documentation
- ISO 13485-certified production facility
- Scalable from milligram to multi-gram quantities
- Consistent lot-to-lot activity specifications
▶ Glycerol-Free & Custom Formulation
Glycerol-free enzyme formats are available for applications requiring lyophilization compatibility, liquid handling automation, or direct IVD master mix integration.
- Glycerol-free liquid format (standard and custom buffers)
- Lyophilization-ready enzyme preparation
- Custom reaction buffer optimization for specific assay conditions
- Compatible with freeze-drying workflows for point-of-care formats
▶ Molecular IVD RDC Service
Yeasen's Research and Development Contracting (RDC) team delivers end-to-end solutions for molecular diagnostic product development, covering enzyme selection through clinical validation support.
- Enzyme selection and performance matching
- Primer/probe design and reaction buffer optimization
- Sensitivity, specificity, and precision validation studies
- Stability studies and SNP evaluation
- Instrument platform compatibility assessment
▶ ZymeEditor™ Enzyme Engineering
Yeasen's proprietary ZymeEditor™ directed evolution and rational design platform enables the development of custom enzyme variants with tailored performance characteristics not available in off-the-shelf products.
- Directed evolution for enhanced thermostability, processivity, or fidelity
- Rational design for altered substrate specificity or cofactor requirements
- Library screening from Yeasen's proprietary enzyme variant collection
- Scale-up to commercial quantities upon candidate confirmation
ⓘ Customization services are fulfilled by Yeasen Biotechnology. Lead times and minimum order quantities vary by service type. Contact BioHippo for project scoping and pricing.
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