| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human Caspase 3 was used as the immunogen for the CASP3 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
CASP3 Antibody / Caspase 3 is a anti-CASP3 Rabbit antibody Recombinant Rabbit Monoclonal clone 30C96 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: CASP3
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 30C96, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC, IP
Biological background
Caspase 3 is synthesized as an inactive zymogen that undergoes proteolytic cleavage during apoptosis to generate the active enzyme. Research using CASP3 antibody has demonstrated that activated Caspase 3 cleaves structural proteins, DNA repair enzymes, and signaling molecules, dismantling cellular architecture and promoting apoptotic progression. Its activity is a hallmark of programmed cell death and is widely used as a biomarker of apoptosis in experimental systems.
In cancer research, studies with CASP3 antibody have revealed that impaired Caspase 3 activation contributes to resistance to chemotherapy and radiation therapy. Tumors that downregulate or inactivate Caspase 3 evade apoptosis, enhancing survival and proliferation. Conversely, forced activation of Caspase 3 promotes cell death and tumor regression. These observations highlight Caspase 3 as a potential therapeutic target and prognostic marker in oncology.
Caspase 3 also plays critical roles in neurodegeneration. Research using CASP3 antibody has shown that aberrant Caspase 3 activity contributes to neuronal loss in diseases such as Alzheimer's, Parkinson's, and Huntington's. Excessive activation promotes neuronal apoptosis, while inhibition has been explored as a neuroprotective strategy. Beyond the nervous system, Caspase 3 regulates apoptosis in immune cells, cardiac cells, and developmental processes, reflecting its broad biological importance.
CASP3 antibody is widely used in western blotting, immunohistochemistry, flow cytometry, and ELISA. Western blotting distinguishes full length and cleaved forms, immunohistochemistry detects apoptotic cells in tissue, flow cytometry quantifies apoptosis in cell populations, and ELISA measures Caspase 3 activity in lysates. These applications make CASP3 antibody indispensable for apoptosis research.
By supplying validated CASP3 antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
