| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human CBX6 recombinant protein (Position: D50-K236) was used as the immunogen for the CBX6 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
CBX6 Antibody / Chromobox protein homolog 6 is a anti-CBX6 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear.
Key elements and design rationale
- Target: CBX6
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC/IF, FACS, ELISA
Biological background
CBX6 binds to trimethylated histone H3 at lysine 27 (H3K27me3), a histone modification mark associated with gene repression, serving as a reader of histone methylation marks and recruiting additional factors to maintain chromatin compaction. It contributes to establishing heritable transcriptional repression, ensuring stable lineage-specific expression patterns in embryonic stem cells and adult tissues. Dysregulation of CBX6 antibody targets, particularly in the Polycomb family, has been associated with aberrant cell proliferation and oncogenic transformation. Overexpression or misexpression of CBX6 has been observed in several cancer types, including hepatocellular carcinoma, prostate cancer, and gliomas, where it may influence epithelial-mesenchymal transition (EMT) and cancer stemness features.
In molecular biology and epigenetic research, CBX6 antibody is widely used for immunofluorescence, chromatin immunoprecipitation (ChIP), and western blot assays to examine Polycomb protein localization and function. It helps distinguish CBX6 from other chromobox homologs (CBX2, CBX4, CBX7, CBX8), each of which has distinct but sometimes overlapping genomic binding profiles and developmental roles. CBX6 has been reported to modulate PRC1 recruitment in concert with histone ubiquitination factors such as RNF2 and BMI1, and to act as a chromatin structural modulator linking histone marks to gene repression. The protein is essential in maintaining pluripotency, regulating HOX gene clusters, and ensuring proper cell fate decisions.
CBX6's gene, CBX6, is located on chromosome 22q13.1. It encodes a protein of approximately 412 amino acids, containing a conserved chromodomain responsible for histone binding and a C-terminal region that mediates protein-protein interactions within the PRC complex. Expression is ubiquitous but higher in testis, placenta, and certain cancerous tissues. Loss-of-function mutations or altered methylation of the CBX6 promoter region can disrupt PRC targeting, influencing gene activation programs and tumor suppressor networks. Studies also show CBX6 modulates long noncoding RNA (lncRNA)-mediated gene silencing, including interactions with lncRNAs such as HOTAIR and MALAT1, further linking CBX6 to chromatin topology and transcriptional memory.
Because of its functional importance, CBX6 serves as a key biomarker in epigenetic and oncogenic signaling studies.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
