| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human CBY2 recombinant protein (Position: E40-V448) was used as the immunogen for the CBY2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
CBY2 Antibody / Chibby 2 is a anti-CBY2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: CBY2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
CBY2 is expressed in ciliated tissues including respiratory epithelium, reproductive organs, and brain ependyma. It plays an essential role in the early stages of ciliogenesis by promoting docking of basal bodies to the plasma membrane and initiating axoneme extension. Disruption of CBY2 function leads to defective ciliogenesis, altered epithelial polarity, and impaired signaling through cilia-dependent pathways such as Hedgehog and Wnt. In reproductive cells, CBY2 contributes to flagellar formation and sperm motility, while in neurons it supports ependymal cilia function crucial for cerebrospinal fluid flow.
The CBY2 antibody is widely used in cell biology and developmental studies to analyze ciliary formation, centrosomal organization, and beta-catenin signaling. Western blot analysis detects a 42 kilodalton band corresponding to full-length CBY2, and immunofluorescence reveals punctate staining at basal bodies marked by gamma-tubulin. In cilia-deficient models, CBY2 expression serves as a marker for disrupted ciliogenesis. The antibody is also useful for exploring CBY2's role in planar cell polarity and coordination of multiciliated cell differentiation.
Mutations or reduced expression of CBY2 have been linked to ciliopathies, infertility, and hydrocephalus, reflecting its vital role in ciliary assembly. The CBY2 antibody provides a powerful tool for investigating these disorders and for mapping ciliary protein networks in epithelial and germline systems.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
