| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of mouse CCR1 was used as the immunogen for the CCR1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
CCR1 Antibody / C-C chemokine receptor type 1 is a anti-CCR1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB) with listed reactivity in Mouse, Rat.
Key elements and design rationale
- Target: CCR1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB
Biological background
CCR1 binds multiple chemokines, including CCL3 (MIP-1alpha), CCL5 (RANTES), and CCL7 (MCP-3), triggering intracellular signaling cascades that guide immune cells toward infection or inflammation sites. Ligand binding activates G protein-mediated signaling through phospholipase C, PI3K, and MAPK pathways, resulting in cytoskeletal rearrangement, integrin activation, and chemotaxis. CCR1 also contributes to immune homeostasis by regulating leukocyte retention and activation in tissue microenvironments.
Structurally, CCR1 consists of seven transmembrane alpha-helices characteristic of GPCRs, with extracellular loops for ligand recognition and an intracellular C-terminal tail that interacts with G proteins and regulatory kinases. CCR1 belongs to the C-C chemokine receptor family, which includes CCR2-CCR5, all mediating overlapping but distinct immune signaling profiles. Co-localization studies show CCR1 residing in lipid rafts and endocytic vesicles, allowing rapid receptor internalization and recycling after ligand stimulation.
Functionally, CCR1 regulates leukocyte trafficking during inflammation, autoimmune response, and hematopoiesis. It is critical for monocyte and neutrophil recruitment to sites of infection and tissue injury. The receptor also participates in bone remodeling by influencing osteoclast differentiation through CCL5 signaling. In the nervous system, CCR1 modulates neuroinflammation by controlling microglial migration and cytokine release.
Dysregulation of CCR1 contributes to various pathological conditions including rheumatoid arthritis, multiple sclerosis, and chronic obstructive pulmonary disease (COPD). Overexpression enhances leukocyte infiltration and tissue inflammation, whereas pharmacological inhibition of CCR1 has shown therapeutic potential in autoimmune and fibrotic disorders. Pathway involvement includes chemokine-mediated signaling, GPCR activation, and NF-kappaB inflammatory pathways. During development, CCR1 expression is upregulated in hematopoietic progenitors and inflammatory cells responding to infection.
The CCR1 antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
