| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human CD116 was used as the immunogen for the CD116 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
CD116 Antibody / CSF2RA / Colony stimulating factor 2 receptor alpha is a anti-CD116 Rabbit antibody Recombinant Rabbit Monoclonal clone 31C12 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human.
Key elements and design rationale
- Target: CD116
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 31C12, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB
Biological background
Colony stimulating factor 2 receptor alpha pairs with a common beta chain, shared with IL-3 and IL-5 receptors, to form a functional heterodimeric receptor. Research using CD116 antibody has demonstrated that ligand binding initiates dimerization and activation of JAK2, which then phosphorylates downstream signaling proteins including STAT5. This signaling cascade regulates survival, proliferation, and activation of granulocytes, macrophages, and eosinophils. The alpha chain provides ligand specificity and determines receptor affinity for granulocyte macrophage colony stimulating factor.
Studies with CD116 antibody have revealed that disruption of CSF2RA function impairs myeloid cell development and immune defense. Loss of function mutations cause X linked pulmonary surfactant metabolism dysfunction, reflecting impaired alveolar macrophage activity. This highlights the critical role of Colony stimulating factor 2 receptor alpha in lung homeostasis and host defense. Conversely, overactivation of the receptor pathway has been implicated in inflammatory diseases, such as asthma and autoimmune disorders.
In cancer biology, CD116 has been linked to leukemia and myelodysplastic syndromes. Research using CD116 antibody has shown that abnormal signaling through the CSF2RA pathway contributes to malignant transformation and proliferation of hematopoietic cells. Because of this, receptor signaling is being explored as a therapeutic target in hematologic malignancies. Monitoring expression with CD116 antibody provides important diagnostic and prognostic information.
CD116 antibody is widely used in flow cytometry, immunohistochemistry, and western blotting. Flow cytometry quantifies receptor expression on myeloid subsets, immunohistochemistry localizes receptor distribution in tissues, and western blotting confirms receptor size and regulation. These applications make CD116 antibody a versatile tool in immunology and hematology research.
By providing validated CD116 antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
