| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | Purified recombinant mouse OX40 antigen was used as the immunogen for the CD134 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
CD134 is a type I integral membrane protein. This receptor is expressed on activated CD4+and CD8+T cells and B cells. The CD134 binds to CD134 ligand (CD252) to provide a costimulatory signal that is independent of CD28. CD134 is involved in coordinating CD4 T cell selection, migration and cytokine differentiation in T helper (Th)1 and Th2 cells. CD134 is also involved in the stimulation of T cells, T dependent humoral response and generation of optimal CD4+ T cell responses in vivo and in vitro. CD134 is expressed on activated CD4+ T lymphocytes, and its ligand, CD134L, is found preferentially on activated B cells. Engagement of CD134 with its ligand, CD134L, delivers a strong costimulatory signal to effector T cells.
This anti-OX40 antibody is supplied as Purified (Rat, Monoclonal (rat origin), clone OX-86, Rat IgG1, kappa, Unconjugated) and is designed to support common target-detection workflows after the on-page specifications.
Key elements and design rationale
- Target: OX40
- Format: Purified
- Species reactivity: Mouse
- Applications (listed): FACS, IF
- Conjugate: Unconjugated
- Clone and antibody class: Monoclonal (rat origin), clone OX-86, Rat IgG1, kappa
Because antibody performance can depend on epitope context, sample preparation, and biological state, interpret signals using appropriate controls and orthogonal evidence when possible.
Biological background
OX40 is referenced in public gene/protein resources (e.g., UniProt and NCBI Gene), which provide curated names/synonyms, protein features, and pathway context. When designing assays, consider potential isoforms, post-translational modifications, and cell-type specific expression that may influence observed signal.
Research relevance and current trends
- Profiling OX40 expression across model systems, perturbations, and time points to support mechanistic hypotheses.
- Combining antibody-based detection with multi-omics or imaging readouts to link OX40 signal with phenotype.
- Using well-matched controls (isotype controls, genetic perturbations, or independent reagents) to strengthen interpretation of target-associated signal.
Common research applications
- FACS
- IF
Use the listed applications as a starting point and tailor experimental design to your sample type and readout requirements.
Notes for experimental interpretation
- Specificity considerations: closely related family members, isoforms, or PTMs can affect apparent specificity; confirm with independent approaches when critical.
- Controls: include negative controls and, when feasible, genetic or pharmacologic perturbations to support target attribution in your system.
- Species and sample context: differences in sequence, expression, fixation, or extraction conditions can change signal behavior across models.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
