| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human CD163 was used as the immunogen for the CD163 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
CD163 Antibody is a anti-CD163 Rabbit antibody Recombinant Rabbit Monoclonal clone 32C15 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC) with listed reactivity in Human.
Key elements and design rationale
- Target: CD163
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 32C15, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC
Biological background
CD163 antibody is widely applied in immunology, hematology, and inflammation research. CD163 expression serves as a marker of alternatively activated macrophages, often associated with tissue repair, immune suppression, and tumor progression. By detecting CD163, researchers can assess macrophage polarization and function in contexts such as sepsis, atherosclerosis, cancer, and chronic inflammation.
Flow cytometry with CD163 antibody distinguishes macrophage populations based on activation state. Immunohistochemistry maps CD163 expression in tissues including liver, spleen, and tumors, while immunofluorescence highlights surface localization on macrophages. Western blotting provides complementary confirmation of expression levels. These applications make CD163 antibody versatile for both basic and translational research.
CD163 has clinical relevance as a biomarker for inflammation and disease activity. Soluble CD163, shed from the cell surface, is detectable in plasma and serves as a marker of macrophage activation. Elevated levels correlate with disease severity in inflammatory conditions such as rheumatoid arthritis, lupus, and sepsis. By applying CD163 antibody, scientists can evaluate macrophage biology and disease mechanisms.
Beyond immunology, CD163 contributes to iron metabolism by mediating hemoglobin clearance. It also influences tumor microenvironments by supporting immune evasion. These diverse functions emphasize the broad significance of CD163 antibody in biology and medicine.
CD163 antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
